关键信息
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基因名
Grb7
- 应用
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别名
Grb7；Growth factor receptor-bound Protein 7
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q14451
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表达区间
1-532aa
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氨基酸序列
MELDLSPPHL SSSPEDLCPA PGTPPGTPRP PDTPLPEEVK RSQPLLIPTT GRKLREEERR ATSLPSIPNP FPELCSPPSQ SPILGGPSSA RGLLPRDASR PHVVKVYSED GACRSVEVAA GATARHVCEM LVQRAHALSD ETWGLVECHP HLALERGLED HESVVEVQAA WPVGGDSRFV FRKNFAKYEL FKSSPHSLFP EKMVSSCLDA HTGISHEDLI QNFLNAGSFP EIQGFLQLRG SGRKLWKRFF CFLRRSGLYY STKGTSKDPR HLQYVADVNE SNVYVVTQGR KLYGMPTDFG FCVKPNKLRN GHKGLRIFCS EDEQSRTCWL AAFRLFKYGV QLYKNYQQAQ SRHLHPSCLG SPPLRSASDN TLVAMDFSGH AGRVIENPRE ALSVALEEAQ AWRKKTNHRL SLPMPASGTS LSAAIHRTQL WFHGRISREE SQRLIGQQGL VDGLFLVRES QRNPQGFVLS LCHLQKVKHY LILPSEEEGR LYFSMDDGQT RFTDLLQLVE FHQLNRGILP CLLRHCCTRV AL
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Grb7 (Growth factor receptor-bound protein 7) is a crucial adapter protein that plays a significant role in various cellular processes, including cell proliferation, migration, and survival, primarily through its interactions with receptor tyrosine kinases. As a member of the Grb7 family, Grb7 has been implicated in cancer progression, particularly in breast and ovarian cancers, where it is often overexpressed. The understanding of Grb7's function and its downstream signaling pathways is essential for developing targeted therapies against cancers that exhibit Grb7 dysregulation. Due to its central role in oncogenic signaling, researchers have focused on characterizing recombinant Grb7 proteins to study their biochemical properties, binding affinities, and interactions with other signaling molecules. The production of recombinant Grb7 enables detailed in vitro and in vivo studies, facilitating the identification of potential drug targets and therapeutic strategies. Furthermore, exploring the molecular mechanisms through which Grb7 mediates its effects on cell signaling can provide insights into the complexity of tumor biology. As investigations into Grb7 continue, the potential to enhance treatment modalities for cancer patients by targeting Grb7-related pathways highlights the importance of ongoing research in this area, paving the way for novel therapeutic interventions and improved clinical outcomes.
