| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | G2/mitotic-specific cyclin-B2, Ccnb2, Cycb2 |
| Entrez GeneID | 12442 |
| WB Predicted band size | 45.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This mouse CCNB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S392 of mouse CCNB2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-mouse CCNB2(S392)抗体的3篇文献示例(内容为模拟，需根据实际文献调整)：
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1. **文献名称**：*Phosphorylation of Cyclin B2 at Serine 392 Regulates Mitotic Progression in Mouse Oocytes*
**作者**：Zhang Y, et al.
**摘要**：研究揭示了在小鼠卵母细胞中，CCNB2的S392位点磷酸化通过调控CDK1活性影响纺锤体组装和染色体分离，磷酸化抗体被用于验证体内外功能。
2. **文献名称**：*DNA Damage-Induced Phosphorylation of CCNB2 Serine 392 Promotes G2/M Checkpoint Arrest*
**作者**：Li H, Wang X.
**摘要**：发现DNA损伤通过ATM/ATR激酶诱导CCNB2 S392磷酸化，从而延迟细胞周期进入有丝分裂，抗体用于检测磷酸化水平与检查点活性的相关性。
3. **文献名称**：*Role of CCNB2 Phosphorylation in Meiotic Resumption of Mouse Germ Cells*
**作者**：Chen R, et al.
**摘要**：利用Phospho-S392抗体证实该位点的磷酸化在减数分裂重启中受PLK1调控，并影响细胞周期蛋白复合物的稳定性。
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**注意**：以上文献为示例，实际需通过PubMed或Google Scholar检索真实文献(关键词：CCNB2 phosphorylation Ser392 mouse)。若研究较少，可扩展至哺乳动物同源位点或相关通路的研究。
The Phospho-mouse CCNB2(S392) antibody is a specialized tool for detecting the phosphorylated form of cyclin B2 (CCNB2) at serine 392 in mouse-derived samples. Cyclin B2. a regulatory subunit of the cyclin-dependent kinase 1 (CDK1), plays a critical role in controlling the G2/M transition and mitotic progression during the cell cycle. Phosphorylation at specific residues, such as S392. is essential for regulating CCNB2 activity, localization, and interaction with CDK1. This post-translational modification is tightly linked to cell cycle checkpoints, ensuring proper DNA replication and error-free chromosome segregation.
The antibody is particularly useful in studies investigating cell cycle dynamics, mitotic regulation, and DNA damage responses. By targeting the phosphorylated S392 epitope, it enables researchers to assess the activation status of CCNB2 during specific phases of the cell cycle or under stress conditions. Its applications include Western blotting, immunofluorescence, and immunohistochemistry, providing insights into dysregulated cell cycle mechanisms in diseases like cancer. Validation of this antibody typically involves testing in knockout models or phosphatase-treated samples to confirm phosphorylation-specific binding. Understanding CCNB2 phosphorylation at S392 may contribute to identifying biomarkers or therapeutic targets for conditions marked by uncontrolled proliferation.
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