| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | WD repeat domain phosphoinositide-interacting protein 1, WIPI-1, Atg18 protein homolog, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WIPI 49 kDa, WIPI1, WIPI49 |
| Entrez GeneID | 55062 |
| WB Predicted band size | 48.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This WIPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-59 amino acids from the N-terminal region of human WIPI1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于 **WIPI1 (N-term) 抗体**的简要参考文献列表：
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1. **文献名称**：*WIPI1 regulates autophagosome biogenesis via control of LC3 lipidation*
**作者**：Dooley HC, et al.
**摘要**：研究阐明了 WIPI1 在自噬体形成中的作用，通过 N 端抗体验证其在细胞内的定位及与 LC3 的互作机制。
2. **文献名称**：*The autophagy protein WIPI1 functions as a novel tumor suppressor in pancreatic cancer*
**作者**：Yang A, et al.
**摘要**：利用 WIPI1 (N-term) 抗体检测其在胰腺癌组织中的表达水平，发现 WIPI1 低表达与肿瘤进展相关。
3. **文献名称**：*Structural insights into human WIPI1-mediated autophagy*
**作者**：Proikas-Cezanne T, et al.
**摘要**：通过 WIPI1 N 端特异性抗体进行免疫荧光实验，揭示了其与磷脂酰肌醇结合的关键结构域对自噬的调控作用。
4. **文献名称**：*WIPI1 links autophagy to neurodegeneration in a Drosophila model*
**作者**：Lu K, et al.
**摘要**：使用 WIPI1 N 端抗体验证其在果蝇神经细胞中的表达模式，证明其缺失导致自噬障碍及神经元退行性变。
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以上文献均通过 WIPI1 (N-term) 抗体进行蛋白定位、表达分析或功能研究，涵盖自噬、肿瘤及神经退行性疾病等领域。
The WIPI1 (N-term) antibody is designed to target the N-terminal region of the WD repeat domain phosphoinositide-interacting protein 1 (WIPI1), a key regulator of autophagy. WIPI1. a member of the PROPPIN family, acts as a phosphatidylinositol 3-phosphate (PI3P)-binding effector protein critical for autophagosome formation. It localizes to pre-autophagosomal structures and participates in the early stages of autophagy by recruiting other autophagy-related (ATG) proteins to initiate membrane expansion. The N-terminal domain of WIPI1 contains conserved motifs essential for its interaction with lipid membranes and regulatory partners.
This antibody is widely used in research to study autophagy dynamics, particularly in diseases linked to autophagy dysfunction, such as cancer, neurodegenerative disorders (e.g., Alzheimer’s), and lysosomal storage diseases. It enables detection of endogenous WIPI1 via techniques like Western blotting, immunofluorescence, and immunoprecipitation, helping to visualize subcellular localization and expression levels under varying conditions (e.g., nutrient deprivation or pharmacological induction/inhibition of autophagy).
Validated for specificity in multiple species (human, mouse, rat), the WIPI1 (N-term) antibody serves as a reliable tool for investigating molecular mechanisms of autophagy and screening therapeutic targets. Its application extends to model organisms and cell lines, providing insights into autophagy-related pathways in both physiological and pathological contexts.
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