| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HIV Tat-specific factor 1;HIV-1 Tat specific factor 1 |
| WB Predicted band size | 25.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HTATSF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-179 amino acids from the C-terminal region of human HTATSF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于HTATSF1抗体的3篇参考文献示例(注：部分文献为虚构示例，实际引用时请核实真实来源)：
1. **文献名称**：*"HTATSF1 is essential for pre-mRNA splicing and cell viability in mammalian cells"*
**作者**：Chen, L., et al.
**摘要**：本研究利用特异性HTATSF1抗体，通过免疫沉淀和RNA-seq分析，揭示了HTATSF1在pre-mRNA剪接中的核心作用，并证明其缺失导致细胞周期停滞和凋亡。
2. **文献名称**：*"Development of a monoclonal antibody against HTATSF1 for detecting its expression in human cancers"*
**作者**：Wang, Y., et al.
**摘要**：报道了一种新型抗HTATSF1单克隆抗体的开发与验证，该抗体在免疫组化(IHC)和Western blot中表现出高特异性，并在多种癌症组织中检测到HTATSF1的异常高表达。
3. **文献名称**：*"HTATSF1 interacts with HIV-1 Tat protein to modulate viral transcription"*
**作者**：Garcia, M., et al.
**摘要**：通过HTATSF1抗体进行共免疫沉淀实验，发现HTATSF1与HIV-1 Tat蛋白直接互作，调控病毒转录活性，为抗病毒治疗提供潜在靶点。
如需真实文献，建议通过PubMed或Google Scholar检索关键词(如“HTATSF1 antibody”、“HTATSF1 function”)，并筛选涉及抗体应用的实验研究。
The HTATSF1 (HIV-1 Tat-specific factor 1) antibody is a tool used to study the HTATSF1 protein, a cellular cofactor critical for HIV-1 replication and transcriptional regulation. HTATSF1. also known as Tat-SF1. interacts with the HIV-1 Tat protein to enhance viral transcription elongation by recruiting components of the RNA polymerase II machinery and splicing factors. Beyond virology, HTATSF1 is involved in cellular mRNA processing, including transcription-coupled splicing and 3' end formation. It plays roles in cell cycle progression, apoptosis, and development, with dysregulation linked to cancers and neurodevelopmental disorders.
HTATSF1 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions. Validation often includes knockout cell lines or siRNA knockdown to confirm specificity. Commercial antibodies are available from suppliers like Abcam, Santa Cruz Biotechnology, and Cell Signaling Technology. Research utilizing HTATSF1 antibodies has advanced understanding of host-pathogen interactions and RNA metabolism, though challenges remain in characterizing its isoform-specific functions. These reagents are essential for probing HTATSF1's dual roles in viral pathogenesis and cellular homeostasis.
×
