Rabbit Polyclonal MouseIrf3(N-term) Antibody

The Mouse Irf3 (N-term) antibody is a specific immunological tool designed to detect the N-terminal region of interferon regulatory factor 3 (IRF3) in mouse-derived samples. IRF3. a transcription factor in the IRF family, plays a pivotal role in innate immune responses, particularly in antiviral signaling pathways. Upon viral infection or pattern recognition receptor (PRR) activation (e.g., TLR3. RIG-I), IRF3 undergoes phosphorylation and conformational changes, leading to its dimerization and nuclear translocation. In the nucleus, IRF3 binds to interferon-stimulated response elements (ISREs) to drive the expression of type I interferons (IFN-α/β) and proinflammatory cytokines. The N-terminal domain of IRF3 contains critical functional motifs, including the DNA-binding domain and regulatory regions essential for its activation and interaction with cofactors like TBK1 or IKKε. Antibodies targeting this region are widely used in applications such as Western blotting, immunofluorescence, and immunoprecipitation to study IRF3 expression, post-translational modifications (e.g., phosphorylation at Ser386/Ser396), and subcellular localization during immune activation. Their specificity makes them valuable for investigating IRF3's role in viral infections, autoimmune diseases, and cancer immunology, as well as validating IRF3 knockout or knockdown models. Proper controls (e.g., IRF3-deficient cells) are recommended to confirm antibody specificity due to potential cross-reactivity with other IRF family members.