WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Interleukin enhancer-binding factor 2, Nuclear factor of activated T-cells 45 kDa, ILF2, NF45 |
Entrez GeneID | 3608 |
WB Predicted band size | 43.1kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This ILF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-112 amino acids from the N-terminal region of human ILF2. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ILF2 (N-term)抗体的3篇参考文献示例(注:文献为模拟示例,实际需通过学术数据库验证):
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1. **文献名称**: *ILF2 regulates DNA repair and antibody diversification in B cells*
**作者**: Smith A, et al.
**摘要**: 研究利用针对ILF2 N端区域的抗体,通过免疫沉淀和ChIP-seq技术,揭示了ILF2在B细胞DNA损伤修复和抗体类别转换重组中的调控作用,证明其N端结构域对结合RNA和DNA至关重要。
2. **文献名称**: *NF45/ILF2 interacts with viral RNA to promote hepatitis B virus replication*
**作者**: Chen L, et al.
**摘要**: 通过Western blot和免疫荧光(使用ILF2 N-term特异性抗体),发现ILF2通过N端结构域与乙肝病毒(HBV)RNA结合,增强病毒复制,为抗病毒治疗提供潜在靶点。
3. **文献名称**: *A novel role of ILF2 in alternative splicing regulation*
**作者**: Kim J, et al.
**摘要**: 该研究使用ILF2 N端抗体进行蛋白质互作分析,发现ILF2通过N端与剪接因子相互作用,调控前体mRNA的选择性剪接,影响癌症相关基因表达。
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如需具体文献,建议在PubMed或Google Scholar中检索关键词:**"ILF2 antibody N-terminal"** 或 **"anti-ILF2 (N-term)"**,并筛选实验方法中明确使用该抗体的研究。部分抗体公司(如Abcam、CST)的产品说明书也会列出引用文献。
The ILF2 (N-term) antibody targets the N-terminal region of Interleukin Enhancer Binding Factor 2 (ILF2), a protein involved in diverse cellular processes. ILF2. also known as NFAR1 or NF45. forms a heterodimer with ILF3 (NF90/NF110) to regulate RNA metabolism, including mRNA stability, nuclear export, and translation. This complex plays roles in transcriptional regulation, DNA damage repair, and maintaining genomic stability. ILF2 is implicated in immune responses, viral infection pathways, and cell cycle control, with dysregulation linked to cancers, autoimmune diseases, and neurological disorders.
The ILF2 (N-term) antibody is commonly used to detect full-length ILF2 protein or specific isoforms in applications like Western blotting, immunoprecipitation, and immunofluorescence. Its specificity for the N-terminal domain helps distinguish ILF2 from related proteins or splice variants. Researchers employ this antibody to study ILF2's subcellular localization, expression levels in pathological conditions, and interactions with binding partners. Given ILF2's emerging role as a potential biomarker or therapeutic target in oncology, this antibody also supports investigations into cancer mechanisms and drug development. Validation in relevant species and experimental models is critical to ensure reliability.
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