| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger protein 394, Zinc finger protein with KRAB and SCAN domains 14, ZNF394, ZKSCAN14 |
| Entrez GeneID | 84124 |
| WB Predicted band size | 64.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ZNF394 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-316 amino acids from the Central region of human ZNF394. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZNF394抗体的模拟参考文献示例(注：由于ZNF394研究较为小众，实际文献可能有限，以下内容为假设性示例，建议通过专业数据库核实)：
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1. **文献名称**: *ZNF394 modulates transcriptional regulation in non-small cell lung cancer via histone modification*
**作者**: Li X, Zhang Y, et al.
**摘要**: 本研究利用ZNF394抗体通过染色质免疫沉淀测序(ChIP-seq)技术，揭示了ZNF394在非小细胞肺癌中通过结合特定基因启动子区域调控组蛋白修饰(如H3K4me3)，影响肿瘤细胞增殖的分子机制。
2. **文献名称**: *Development and validation of a polyclonal antibody against human ZNF394 for immunohistochemical applications*
**作者**: Wang H, Chen J, et al.
**摘要**: 文章描述了兔源多克隆ZNF394抗体的制备与验证，通过Western blot和免疫组织化学(IHC)证实其特异性。该抗体成功应用于肝癌组织样本，发现ZNF394在肿瘤边缘区高表达，提示其潜在临床意义。
3. **文献名称**: *ZNF394 interacts with p53 to regulate oxidative stress response in colorectal cancer cells*
**作者**: Kim S, Park M, et al.
**摘要**: 研究通过免疫共沉淀(Co-IP)结合ZNF394抗体，发现ZNF394与p53蛋白相互作用，共同调控结直肠癌细胞氧化应激反应通路。敲低ZNF394可增强化疗药物诱导的细胞凋亡。
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**建议**：
- 实际检索时，可尝试在 **PubMed** 或 **Google Scholar** 中搜索关键词“ZNF394 antibody”或“ZNF394 AND (immunoblotting OR IHC)”;
- 部分抗体厂商(如Abcam、CST)的产品页面可能提供相关引用文献;
- 若研究较少，可扩展至ZNF蛋白家族功能或相关疾病通路的研究。
**Background of ZNF394 Antibody**
ZNF394 (Zinc Finger Protein 394) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, which plays roles in transcriptional regulation and chromatin remodeling. This protein is characterized by an N-terminal KRAB domain, known to mediate protein-protein interactions and recruit co-repressors, and multiple C-terminal zinc finger motifs that facilitate DNA binding. ZNF394 has been implicated in regulating gene expression during development and cellular differentiation, though its precise biological functions remain less explored compared to other zinc finger proteins.
Antibodies targeting ZNF394 are primarily used as research tools to investigate its expression patterns, subcellular localization, and molecular interactions. Studies employing ZNF394 antibodies (e.g., via Western blotting, immunohistochemistry, or immunofluorescence) have detected its presence in nuclear compartments, consistent with its role in transcriptional regulation. Recent research suggests ZNF394 may influence pathways linked to cancer progression, such as in acute myeloid leukemia (AML) and solid tumors, by modulating oncogenic or tumor-suppressive genes.
The development and validation of ZNF394 antibodies are critical for elucidating its mechanistic contributions to diseases and normal cellular processes. Challenges include ensuring antibody specificity due to structural similarities among zinc finger proteins. Ongoing research aims to clarify ZNF394's regulatory networks and its potential as a therapeutic target or biomarker.
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