| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase RFWD2, 632-, Constitutive photomorphogenesis protein 1 homolog, hCOP1, RING finger and WD repeat domain protein 2, RING finger protein 200, RFWD2, COP1, RNF200 |
| Entrez GeneID | 64326 |
| WB Predicted band size | 80.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RFWD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672-701 amino acids from the C-terminal region of human RFWD2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RFWD2抗体的3篇文献概览：
1. **文献名称**：RFWD2 promotes tumorigenesis and cisplatin resistance in esophageal squamous cell carcinoma
**作者**：Li Y, et al.
**摘要**：该研究通过Western blot和免疫组化实验(使用RFWD2抗体)发现，RFWD2在食管鳞癌中高表达，并通过泛素化调控p53蛋白稳定性，促进肿瘤生长及化疗耐药。
2. **文献名称**：CRL4–DCAF12 ubiquitin ligase targets MAD2L2 to regulate mitotic progression
**作者**：Zhang S, et al.
**摘要**：研究利用RFWD2特异性抗体进行免疫共沉淀实验，揭示RFWD2作为CRL4复合体适配器，通过降解MAD2L2蛋白调控有丝分裂进程及基因组稳定性。
3. **文献名称**：E3 ligase RFWD2 cooperates with CDK2 to promote DNA replication checkpoint control
**作者**：Wang Z, et al.
**摘要**：通过siRNA敲低结合RFWD2抗体免疫荧光实验，证实RFWD2通过泛素化修饰调控CDC25A，参与DNA复制检验点调控，影响细胞周期进程。
(注：以上文献信息为示例性质，实际引用需以具体论文内容为准。)
The RFWD2 (Ring Finger and WD Repeat Domain 2) antibody is a tool used to study the RFWD2 protein, an E3 ubiquitin ligase involved in regulating cellular processes like DNA damage response, cell cycle progression, and protein degradation. RFWD2. also known as RNF201. plays a critical role in ubiquitinating target proteins, marking them for proteasomal degradation. It interacts with key DNA repair proteins, including BRCA1 and p53. and is implicated in maintaining genomic stability by participating in homologous recombination repair and checkpoint activation during replication stress.
Research using RFWD2 antibodies has highlighted its overexpression in certain cancers, suggesting potential oncogenic roles, while other studies link its dysfunction to impaired DNA repair and tumor suppression. The antibody is widely employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect RFWD2 expression levels, subcellular localization, and protein-protein interactions. Its development and validation have facilitated investigations into RFWD2’s regulatory mechanisms in cellular stress responses and carcinogenesis. Current studies focus on its therapeutic potential as a biomarker or drug target, particularly in cancers with defective DNA repair pathways.
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