| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 3-ketoacyl-CoA thiolase, peroxisomal, Acetyl-CoA acyltransferase, Beta-ketothiolase, Peroxisomal 3-oxoacyl-CoA thiolase, ACAA1, ACAA, PTHIO |
| Entrez GeneID | 30 |
| WB Predicted band size | 44.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ACAA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-176 amino acids from the Central region of human ACAA1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ACAA1抗体的示例参考文献(注：以下为模拟内容，实际文献请通过学术数据库查询)：
1. **"Characterization of ACAA1-specific antibodies for peroxisomal studies"**
*作者：Smith J, et al.*
*摘要*：本研究开发并验证了针对ACAA1蛋白的多克隆抗体，通过Western blot和免疫荧光技术确认其在人肝细胞系中对过氧化物酶体定位的特异性，为研究ACAA1功能提供了工具。
2. **"ACAA1 enzyme activity and its role in fatty acid oxidation"**
*作者：Lee H, et al.*
*摘要*：利用特异性抗体分析ACAA1在小鼠肝脏中的表达及酶活性，发现其缺失导致β-氧化代谢异常，提示ACAA1在脂代谢中的关键作用。
3. **"ACAA1 as a biomarker in metabolic disorders"**
*作者：Garcia R, et al.*
*摘要*：通过免疫组化技术，发现ACAA1在肥胖患者脂肪组织中表达显著下调，提示其可能成为代谢综合征的潜在生物标志物，研究采用商业化ACAA1抗体进行验证。
4. **"Development of a monoclonal antibody targeting human ACAA1"**
*作者：Wang Y, et al.*
*摘要*：报道了一种高亲和力抗ACAA1单克隆抗体的制备，应用于ELISA和流式细胞术，成功检测了多种癌细胞系中ACAA1的差异表达。
**注意**：以上文献信息为示例，实际文献需通过PubMed、Google Scholar等平台检索。建议结合关键词“ACAA1 antibody”“peroxisomal ACAA1”或“acetyl-CoA acyltransferase 1”查找最新研究。
The ACAA1 antibody is a immunological tool designed to detect acetyl-CoA acyltransferase 1 (ACAA1), a key enzyme in fatty acid metabolism. ACAA1. also known as thiolase 1. is a mitochondrial matrix protein that catalyzes the final step of mitochondrial β-oxidation, converting 3-ketoacyl-CoA into acetyl-CoA and a shortened acyl-CoA. This enzyme plays a critical role in energy production, particularly during prolonged fasting or high-fat metabolism. Dysregulation of ACAA1 has been implicated in metabolic disorders, neurodegenerative diseases, and certain cancers, making it a subject of interest in biomedical research.
ACAA1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study protein expression, subcellular localization, and tissue distribution. They help researchers investigate ACAA1's role in cellular energy homeostasis, lipid metabolism, and disease mechanisms. Validated antibodies typically target specific epitopes, such as the N-terminal region, and are produced in host species like rabbits or mice. Quality-controlled batches undergo rigorous testing for specificity using knockout cell lines or tissue samples. Recent studies have explored ACAA1's potential as a biomarker in metabolic syndrome and its interaction with peroxisome proliferator-activated receptors (PPARs), highlighting the antibody's utility in both basic research and therapeutic development.
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