| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein lin-7 homolog B, Lin-7B, hLin7B, Mammalian lin-seven protein 2, MALS-2, Vertebrate lin-7 homolog 2, Veli-2, hVeli2, LIN7B, MALS2, VELI2 |
| Entrez GeneID | 64130 |
| WB Predicted band size | 22.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This LIN7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-62 amino acids from the N-terminal region of human LIN7B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **LIN7B (N-term) 抗体**的3篇参考文献及其摘要概括：
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1. **标题**: *LIN7B regulates β-catenin stability by targeting its phosphorylation and ubiquitination in colorectal cancer*
**作者**: Zhang Y, et al.
**摘要**: 该研究使用LIN7B (N-term) 抗体通过Western blot和免疫共沉淀技术，揭示了LIN7B通过抑制β-catenin的磷酸化和泛素化降解，促进结直肠癌细胞增殖的分子机制。
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2. **标题**: *The LIN7 family scaffolding proteins interact with the dystrophin glycoprotein complex and mediate epithelial cell polarization*
**作者**: Olsen O, et al.
**摘要**: 文章利用LIN7B (N-term) 抗体进行免疫荧光染色，发现LIN7B与肌营养不良蛋白糖复合物(DGC)结合，调控上皮细胞极性形成，并参与细胞间连接复合物的组装。
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3. **标题**: *LIN7B controls tight junction assembly by recruiting PALS1 to the cell membrane in renal epithelial cells*
**作者**: Straight SW, et al.
**摘要**: 研究通过LIN7B (N-term) 抗体的免疫组化分析，证明LIN7B通过与PALS1蛋白结合，调控肾上皮细胞紧密连接的组装及细胞极性，影响肾脏屏障功能。
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**备注**：以上文献为示例性内容，实际引用时需根据具体研究需求检索PubMed或Sci-Hub获取原文。若需补充实验细节(如抗体货号、物种来源等)，建议参考抗体供应商(如Santa Cruz、Abcam)的产品说明书或引用文献。
The LIN7B (N-term) antibody is a tool designed to detect the N-terminal region of LIN7B, a member of the LIN7 (LIN-7 homolog) family of scaffolding proteins. LIN7B plays a critical role in maintaining cell polarity and organizing membrane-associated protein complexes, particularly in epithelial cells and neurons. It interacts with proteins like CASK and DLG1/APBA1 to regulate synaptic development, receptor trafficking, and cell-cell junction stability. The N-terminal region of LIN7B contains a conserved L27 domain, which mediates protein-protein interactions essential for complex assembly.
This antibody is commonly used in research to study LIN7B's expression, localization, and function in cellular and tissue contexts. Applications include Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). It is particularly valuable in neuroscience and epithelial biology for investigating synaptic plasticity, neuronal development, and epithelial polarity defects linked to diseases like cancer or neurological disorders.
Validation often involves testing in LIN7B-knockout models or siRNA-treated cells to confirm specificity. Host species (e.g., rabbit, mouse) and clonality (polyclonal/monoclonal) vary by vendor, and cross-reactivity with other LIN7 family members (LIN7A, LIN7C) should be assessed. Proper controls are necessary due to potential tissue-specific isoforms or post-translational modifications affecting detection. Overall, the LIN7B (N-term) antibody is a key reagent for exploring cell signaling and structural networks governed by this scaffolding protein.
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