| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DnaJ homolog subfamily C member 25, DNAJC25 |
| Entrez GeneID | 548645;552891 |
| WB Predicted band size | 42.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DNAJC25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the Central region of human DNAJC25. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是假设性的参考文献示例，供参考。请注意，DNAJC25可能并非标准基因符号，建议核实名称准确性：
1. **标题**：DNAJC25 Antibody Characterization in Stress Response Pathways
**作者**：Smith A, et al.
**摘要**：该研究开发了针对DNAJC25中心区域的多克隆抗体，验证其在免疫印迹中的特异性，并发现该蛋白在热休克应激下与Hsp70相互作用，调控细胞存活。
2. **标题**：Role of DNAJC25 in Neuronal Protein Aggregation
**作者**：Lee B, et al.
**摘要**：通过使用抗DNAJC25(Center)抗体，作者发现该蛋白在阿尔茨海默病模型中参与tau蛋白的折叠，抑制其表达加剧神经元内聚集。
3. **标题**：DNAJC25 as a Biomarker in Colorectal Cancer
**作者**：Chen L, et al.
**摘要**：研究利用抗DNAJC25抗体检测肿瘤组织，发现其高表达与患者预后不良相关，提示其可能通过调控Wnt信号通路促进癌细胞增殖。
4. **标题**：Development of a Monoclonal Antibody Targeting DNAJC25 for Functional Studies
**作者**：Rodriguez M, et al.
**摘要**：文章描述了单克隆抗体的制备流程，证实其适用于免疫荧光和Co-IP，并揭示DNAJC25在线粒体应激反应中的定位变化。
**注意**：以上文献为示例性质，实际研究中可能需替换为真实文献。建议通过PubMed或Google Scholar以正确基因符号检索。
The DNAJC25 antibody targets a protein encoded by the DNAJC25 gene, which belongs to the DNAJ/Hsp40 family of molecular chaperones. These proteins are characterized by a conserved J-domain that facilitates interactions with Hsp70 chaperones, aiding in protein folding, translocation, and degradation. DNAJC25. also referred to as mitochondrial J-domain protein (Mdj2) in some contexts, is implicated in mitochondrial function, though its precise biological role remains under investigation. It is thought to participate in stress response pathways and mitochondrial protein homeostasis, potentially influencing cellular energy metabolism and apoptosis regulation.
Antibodies against DNAJC25 are valuable tools for studying its expression, localization, and interaction networks. They are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore its tissue-specific distribution, subcellular compartmentalization (particularly mitochondrial associations), and dysregulation in disease contexts. Research suggests DNAJC25 may have relevance in neurodegenerative disorders, cancer, and metabolic syndromes, though mechanistic insights are still emerging. Commercial DNAJC25 antibodies are typically raised against central (Center) epitopes to ensure specificity, often validated using knockout controls or siRNA-mediated silencing. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to homologous J-proteins, enabling precise detection in complex biological samples.
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