| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Podocan, PODN, SLRR5A |
| Entrez GeneID | 127435 |
| WB Predicted band size | 69.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PODN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-602 amino acids from the C-terminal region of human PODN. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PODN(C-term)抗体的3篇参考文献示例(内容为虚构，仅供参考)：
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1. **文献名称**: *"Characterization of a Novel PODN C-terminal Antibody in Cardiac Fibrosis Models"*
**作者**: Zhang Y, et al.
**摘要**: 本研究开发并验证了一种靶向PODN蛋白C末端的多克隆抗体，通过Western blot和免疫组化证实其在心脏纤维化模型中特异性识别PODN。结果显示，PODN在纤维化组织中显著上调，提示其可能调控细胞外基质的病理重塑。
2. **文献名称**: *"PODN C-terminal Antibody Reveals Tumor-Suppressive Role in Glioblastoma"*
**作者**: Müller S, et al.
**摘要**: 利用PODN C-term抗体进行免疫沉淀和功能实验，发现PODN通过C末端结构域与整合素相互作用，抑制胶质母细胞瘤细胞的侵袭能力，表明其作为肿瘤抑制因子的潜在机制。
3. **文献名称**: *"Development of a High-Affinity Monoclonal Antibody Against PODN C-terminal for Biomarker Detection"*
**作者**: Kim T, et al.
**摘要**: 报道了一种高亲和力单克隆抗体的制备，特异性靶向PODN的C末端表位。该抗体成功应用于ELISA和流式细胞术，检测到肝硬化患者血清中PODN水平升高，为疾病诊断提供新工具。
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*注：以上文献为模拟示例，实际引用需查询权威数据库(如PubMed、Web of Science)。*
PODN (Podocan) is a member of the small leucine-rich proteoglycan (SLRP) family, which plays critical roles in extracellular matrix (ECM) organization, cell signaling, and tissue homeostasis. The PODN gene encodes a secreted protein predominantly expressed in vascular smooth muscle cells, fibroblasts, and certain immune cells. It is implicated in regulating cell adhesion, migration, and inflammatory responses, with emerging links to cardiovascular diseases, fibrosis, and cancer progression. The C-terminal (C-term) region of Podocan contains conserved structural motifs that mediate protein-protein interactions and ECM integration, making it a target for functional studies.
Antibodies targeting the C-terminal domain of Podocan (PODN(C-term)) are essential tools for investigating its expression, localization, and molecular interactions. These antibodies are typically developed using immunogenic peptides derived from the C-terminal sequence, ensuring specificity for this region. They enable applications such as Western blotting, immunohistochemistry, and immunofluorescence to assess Podocan’s tissue distribution and alterations in disease states. Research using PODN(C-term) antibodies has highlighted its role in suppressing Wnt/β-catenin signaling in vascular calcification and modulating TGF-β activity in fibrotic pathways. Additionally, studies suggest Podocan’s dual role as both a tumor suppressor and promoter, depending on context, underscoring the importance of region-specific antibodies in dissecting its complex functions. Validated PODN(C-term) antibodies are crucial for advancing mechanistic insights into its contributions to ECM dynamics and disease pathogenesis.
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