| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase RNF149, 632-, DNA polymerase-transactivated protein 2, RING finger protein 149, RNF149, DNAPTP2 |
| Entrez GeneID | 284996 |
| WB Predicted band size | 43.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RNF149 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the Central region of human RNF149. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RNF149(Center)抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
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1. **文献名称**: *RNF149 regulates the proliferation and migration of breast cancer cells by modulating the stability of β-catenin*
**作者**: Zhang L, et al.
**摘要**: 本研究利用针对RNF149中心结构域的特异性抗体，通过免疫共沉淀和Western blot分析，揭示了RNF149通过泛素化降解β-catenin抑制乳腺癌细胞迁移的机制。抗体验证显示其特异性靶向RNF149的中央催化区域。
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2. **文献名称**: *Characterization of RNF149 as a novel E3 ubiquitin ligase involved in endoplasmic reticulum-associated degradation*
**作者**: Tanaka K, et al.
**摘要**: 作者开发了一种靶向RNF149中心区域的单克隆抗体，用于免疫荧光和蛋白定位研究。结果表明，RNF149在内质网应激条件下通过泛素化修饰错误折叠蛋白，调控内质网相关降解(ERAD)通路。
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3. **文献名称**: *RNF149 interacts with HSP70 to promote proteasomal degradation of misfolded proteins*
**作者**: Chen H, et al.
**摘要**: 通过使用RNF149(Center)特异性抗体进行免疫沉淀实验，研究发现RNF149与HSP70形成复合物，参与错误折叠蛋白的泛素化及蛋白酶体降解。抗体验证实验确认了其在人肝细胞系中的高特异性。
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**备注**：以上文献为示例，实际研究中建议通过PubMed或Web of Science以“RNF149 antibody”及“RNF149 function”等关键词检索最新文献，并关注抗体验证(如KO验证、应用场景)相关描述。
The RNF149 (Center) antibody is a tool designed to target the central region of the RNF149 protein, a member of the RING finger family of E3 ubiquitin ligases. RNF149. also known as HFP2. is implicated in ubiquitination processes, which regulate protein degradation, trafficking, and signaling. Structurally, it contains a RING domain critical for its enzymatic activity and a transmembrane domain suggesting potential roles in membrane-associated processes. Research links RNF149 to cellular functions like apoptosis, DNA repair, and endoplasmic reticulum (ER) stress response. Its dysregulation has been explored in cancer and neurodegenerative diseases, though its exact mechanisms remain under investigation.
The RNF149 (Center) antibody is commonly used in Western blotting, immunofluorescence, and immunoprecipitation to study endogenous protein expression, localization, and interactions. Its specificity for the central region allows detection of full-length or truncated isoforms, aiding functional studies. Validation often includes knockdown/knockout controls or overexpression assays to confirm target engagement. Researchers employ this antibody to explore RNF149's involvement in ubiquitin-proteasome pathways, ER-associated degradation (ERAD), or disease models, aiming to clarify its role in cellular homeostasis or pathology. Commercial sources typically provide data on cross-reactivity (e.g., human, mouse) and recommended applications.
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