| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Glucose-6-phosphate isomerase, GPI, Autocrine motility factor, AMF, Neuroleukin, NLK, Phosphoglucose isomerase, PGI, Phosphohexose isomerase, PHI, Gpi, Gpi1 |
| Entrez GeneID | 14751 |
| WB Predicted band size | 62.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Gpi antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 532-558 amino acids from the C-terminal region of mouse Gpi. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse GPI(C-term)抗体的3篇参考文献示例，基于相关领域的研究主题整理：
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1. **文献名称**: *Autoantigen glucose-6-phosphate isomerase mediates inflammatory cytokine production in rheumatoid arthritis*
**作者**: Schaller, M., et al.
**摘要**: 该研究利用小鼠GPI(C-term)抗体检测类风湿关节炎模型中的GPI表达，揭示GPI作为自身抗原通过激活NF-κB通路促进炎症因子释放，为治疗靶点提供依据。
2. **文献名称**: *Metabolic role of glucose-6-phosphate isomerase in tumor progression: Insights from antibody-based detection*
**作者**: Xu, L., et al.
**摘要**: 通过C端特异性抗体分析小鼠肿瘤组织中GPI的定位及表达水平，发现GPI在糖酵解重编程中的关键作用，并与肿瘤转移潜能呈正相关。
3. **文献名称**: *Antibody screening for GPI-anchored protein defects in paroxysmal nocturnal hemoglobinuria models*
**作者**: Merkel, P., et al.
**摘要**: 研究采用小鼠GPI(C-term)抗体筛选GPI锚定蛋白缺失细胞，验证其在阵发性夜间血红蛋白尿症(PNH)模型中的应用，为疾病诊断提供新方法。
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**注**: 上述文献为示例性整理，实际引用时需根据具体研究内容查询真实存在的文献，并通过PubMed、Google Scholar等平台核对作者、标题及摘要准确性。
The Mouse GPI (C-term) antibody is designed to detect glycosylphosphatidylinositol (GPI)-anchored proteins in mouse-derived samples by targeting their C-terminal region. GPI anchors are post-translational modifications that tether proteins to cell membranes, playing critical roles in signal transduction, cell adhesion, and membrane trafficking. These anchors are synthesized in the endoplasmic reticulum and attached to proteins, replacing their hydrophobic transmembrane domains. The C-terminal region recognized by this antibody corresponds to the conserved sequence near the GPI attachment site, making it a useful tool for studying GPI-anchored protein expression, processing, or localization.
This antibody is commonly employed in techniques like Western blotting, immunohistochemistry, or flow cytometry to investigate GPI-anchored proteins such as alkaline phosphatase, CD59. or prion proteins. It may also aid in studying diseases linked to GPI biosynthesis defects, including paroxysmal nocturnal hemoglobinuria (PNH) or neurological disorders. Researchers often use it to validate GPI-anchored protein knockdown models or to analyze membrane protein dynamics in lipid rafts. Specificity is typically confirmed using GPI-deficient cell lines or enzymatic cleavage (e.g., phospholipase C treatment). Proper controls are essential, as some cross-reactivity with non-GPI proteins containing similar C-terminal motifs may occur.
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