| WB | 1/1000 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Arginase-1, Liver-type arginase, Type I arginase, Arg1 |
| Entrez GeneID | 29221 |
| WB Predicted band size | 35.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | This Rat Arg1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-318 amino acids from the C-terminal region of rat Arg1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 Rat Arg1 (C-term) 抗体的3篇参考文献示例(注：文献为假设性示例，实际引用需核实)：
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1. **标题**：*Arginase 1 Expression in Rat Hepatic Macrophages during Liver Injury*
**作者**：Munder M, Schneider H, Luckner C.
**摘要**：研究大鼠肝损伤模型中Arg1的表达变化，采用C末端特异性抗体进行免疫组化(IHC)和Western blot分析，发现Arg1在修复期巨噬细胞中显著上调，提示其参与组织修复调控。
2. **标题**：*Regulation of Arginase 1 in Rat Tumor-Associated Myeloid Cells*
**作者**：Morris SM, Kepka-Lenhart D, Chen LC.
**摘要**：通过抗Rat Arg1 (C-term) 抗体检测肿瘤微环境中髓系细胞的Arg1活性，证实其通过抑制T细胞功能促进免疫逃逸，为靶向Arg1的肿瘤治疗提供依据。
3. **标题**：*C-Terminal Epitope-Specific Detection of Arg1 in Rat Neuroinflammation Models*
**作者**：Hesse C, Gielen S, Vandenbroeck K.
**摘要**：利用针对Arg1 C末端的抗体，在大鼠神经炎症脑组织中验证了Arg1在小胶质细胞中的表达，并关联其与神经保护机制的关系。
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**注**：以上文献为模拟示例，实际研究中需通过学术数据库(如PubMed、Web of Science)以关键词“Arg1 C-term antibody rat”检索具体文献，并核对抗体货号及实验方法。
The Rat Arg1 (C-term) antibody is a polyclonal or monoclonal antibody specifically designed to detect the C-terminal region of arginase 1 (Arg1) in rat samples. Arginase 1 is a key enzyme in the urea cycle, primarily expressed in the liver, where it catalyzes the hydrolysis of arginine to ornithine and urea, playing a critical role in nitrogen metabolism. Beyond hepatic function, Arg1 is also implicated in immune regulation, particularly in alternatively activated (M2) macrophages, where it modulates inflammation and tissue repair by depleting arginine, a substrate for nitric oxide synthase.
This antibody is commonly used in research to study Arg1 expression in rat models of liver disease, metabolic disorders, and immune-related conditions. Its specificity for the C-terminal domain ensures recognition of full-length, mature Arg1 protein, minimizing cross-reactivity with truncated isoforms or related proteins like Arg2. Validation typically includes Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), with confirmed reactivity in liver tissue and macrophages.
Researchers employ this tool to explore Arg1's dual roles in detoxification and immune suppression, particularly in pathologies such as fibrosis, cancer, and infection. Its development and optimization often involve immunization with synthetic peptides or recombinant proteins corresponding to the C-terminal sequence, followed by affinity purification to enhance specificity. Proper controls, including knockout validation, are recommended to confirm signal accuracy in experimental settings.
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