| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KH domain-containing, RNA-binding, signal transduction-associated protein 1, GAP-associated tyrosine phosphoprotein p62, Src-associated in mitosis 68 kDa protein, Sam68, p21 Ras GTPase-activating protein-associated p62, p68, Khdrbs1 {ECO:0000312|MGI:MGI:893579} |
| Entrez GeneID | 20218 |
| WB Predicted band size | 48.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Khdrbs1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-443 amino acids from the C-terminal region of mouse Khdrbs1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Khdrbs1抗体的参考文献示例(注：文献为模拟示例，实际引用需根据真实文献调整)：
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1. **文献名称**: *"Sam68 regulates spermatogenesis through RNA metabolism in mouse testes"*
**作者**: Taylor S.J. et al. (2004)
**摘要**: 研究利用小鼠Khdrbs1(Sam68)特异性抗体，通过Western blot和免疫组化分析Sam68在精子发生中的作用，发现其通过结合特定RNA靶点调控减数分裂过程。
2. **文献名称**: *"Khdrbs1/Sam68 is essential for cortical neuron development in mice"*
**作者**: Paronetto M.P. et al. (2007)
**摘要**: 该文献通过制备小鼠单克隆Khdrbs1抗体，验证其在中枢神经系统中的特异性，并揭示Sam68在神经元突触形成和RNA剪接中的关键功能。
3. **文献名称**: *"Characterization of a monoclonal antibody against mouse Sam68 for RNA-binding studies"*
**作者**: Richard S. et al. (2005)
**摘要**: 描述了小鼠Sam68单克隆抗体的开发与验证，证明其在免疫沉淀(IP)和免疫荧光(IF)中的高效性，用于研究RNA-蛋白相互作用机制。
4. **文献名称**: *"Sam68 deficiency alters mammary gland development in mice"*
**作者**: Lukong K.E. & Richard S. (2003)
**摘要**: 使用Khdrbs1抗体检测Sam68蛋白在小鼠乳腺组织中的表达，发现其通过调控信号通路影响乳腺上皮细胞增殖与分化。
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**提示**：实际引用时建议通过PubMed或Google Scholar检索关键词(如“Khdrbs1 antibody mouse”“Sam68 mouse model”)，筛选涉及抗体开发、验证或功能研究的文献。若需具体文献，可提供更详细信息以便精准查找。
The mouse Khdrbs1 antibody is a tool designed to detect and study the Khdrbs1 protein, also known as SAM68 (Src-associated in mitosis 68 kDa), in murine models. Khdrbs1 encodes an RNA-binding protein belonging to the STAR (Signal Transduction and Activation of RNA) family, which regulates RNA metabolism, including splicing, transport, and translation. SAM68 is involved in diverse cellular processes such as cell cycle progression, apoptosis, and signal transduction, particularly in pathways mediated by Src family kinases. It plays roles in neurodevelopment, cancer progression, and immune responses, making it a focus of studies on diseases like glioblastoma, leukemia, and neurodegenerative disorders.
The antibody is typically produced in hosts like rabbits or mice, using immunogens derived from mouse Khdrbs1 protein fragments. It is validated for specificity through techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP), often confirmed by knockout controls. Researchers employ this antibody to investigate SAM68 expression patterns, post-translational modifications (e.g., phosphorylation), and interactions with RNA or proteins in mouse tissues or cell lines. Its application in preclinical models aids in elucidating SAM68's role in cellular stress responses, alternative splicing events, and disease mechanisms, supporting translational research targeting RNA-binding proteins.
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