| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Crk-like protein, Crkl, Crkol |
| Entrez GeneID | 12929 |
| WB Predicted band size | 33.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Crkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-303 amino acids from the C-terminal region of mouse Crkl. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与小鼠Crkl抗体相关的文献示例(文献信息为模拟虚构，仅供格式参考)：
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1. **文献名称**: *Crkl regulates endothelial cell migration and vasculogenesis through SH2 domain interactions*
**作者**: Smith A, et al.
**摘要**: 该研究利用小鼠Crkl特异性抗体，通过免疫沉淀和Western blot技术，揭示了Crkl在内皮细胞迁移和血管生成中通过结合SH2结构域调控下游信号通路的机制。
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2. **文献名称**: *Role of Crkl in neural crest cell development: Insights from mouse knockout models*
**作者**: Chen L, et al.
**摘要**: 通过免疫组织化学(使用小鼠Crkl抗体)和基因敲除模型，作者发现Crkl缺失导致小鼠神经嵴细胞迁移异常，并影响胚胎发育中颅面结构的形成。
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3. **文献名称**: *Crkl-mediated signaling in T-cell receptor activation*
**作者**: Park J, et al.
**摘要**: 研究采用小鼠Crkl抗体进行流式细胞术和共聚焦显微镜分析，证明Crkl在T细胞受体激活过程中通过衔接蛋白复合物传递PI3K/Akt信号，影响免疫应答。
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4. **文献名称**: *Crkl as a biomarker in colorectal cancer: Evidence from murine xenograft studies*
**作者**: Gupta R, et al.
**摘要**: 通过小鼠移植瘤模型和Crkl抗体的免疫印迹分析，研究显示Crkl表达水平与肿瘤侵袭性正相关，提示其作为结直肠癌潜在治疗靶点的可能性。
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(注：以上文献为示例性内容，实际引用时需检索真实数据库如PubMed或Google Scholar获取准确信息。)
The mouse Crkl antibody is a tool used to detect and study the Crk-like (Crkl) protein in murine models. Crkl is a 38-40 kDa adaptor protein belonging to the Crk family, which includes CrkI and CrkII. It contains one SH2 domain and two SH3 domains, enabling interactions with signaling molecules to regulate pathways like RAS-MAPK, PI3K/AKT, and integrin signaling. Crkl plays roles in cell proliferation, migration, adhesion, and apoptosis. Dysregulation of Crkl is linked to cancers, immune disorders, and developmental defects.
In mice, Crkl shares high homology with human CRKL, making it a valuable model for studying human diseases. The antibody targets specific epitopes of mouse Crkl, aiding in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). It helps elucidate Crkl's involvement in pathologies like leukemia, solid tumors, and T-cell signaling anomalies. Researchers also use it to explore Crkl's interaction with downstream effectors (e.g., C3G, SOS) and upstream regulators (e.g., BCR-ABL fusion protein).
Validated antibodies typically undergo specificity tests using knockout controls. Applications extend to developmental biology, given Crkl's role in embryogenesis. Commercial Crkl antibodies may vary in clonality (monoclonal/polyclonal), host species (rabbit, mouse), and conjugate types (e.g., HRP, fluorescent tags). Proper validation ensures reliability in experimental outcomes.
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