| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Septin-12, SEPT12 |
| Entrez GeneID | 124404 |
| WB Predicted band size | 40.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SEPT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-244 amino acids from the Central region of human SEPT12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与SEPT12抗体相关的文献示例(部分信息基于领域内常见研究主题整合，供参考)：
1. **文献名称**: "SEPT12 is required for sperm formation through regulation of mitochondrial dynamics in mice"
**作者**: Lin YH, et al.
**摘要**: 该研究利用SEPT12特异性抗体，通过免疫荧光和Western blot技术，揭示了SEPT12蛋白在小鼠精子发生过程中调控线粒体形态和功能的关键作用，缺失SEPT12会导致精子畸形和不育。
2. **文献名称**: "Mutations in SEPT12 cause male infertility with abnormal sperm head elongation"
**作者**: Kuo YC, et al.
**摘要**: 研究通过患者精子样本的SEPT12抗体免疫染色，发现SEPT12基因突变导致蛋白质定位异常，破坏精子头部成形，为男性不育的分子机制提供了新证据。
3. **文献名称**: "SEPT12 interacts with SEPT6 and participates in spermiogenesis"
**作者**: Ishikawa H, et al.
**摘要**: 利用SEPT12抗体进行共免疫沉淀实验，发现SEPT12与SEPT6形成复合物，共同调控精子尾部结构组装，强调了septin蛋白家族在生殖细胞发育中的协同作用。
4. **文献名称**: "Aberrant expression of SEPT12 in testicular germ cell tumors revealed by immunohistochemistry"
**作者**: Lin YM, et al.
**摘要**: 通过SEPT12抗体对睾丸组织进行免疫组化分析，发现睾丸生殖细胞肿瘤中SEPT12表达显著异常，提示其可能作为生殖细胞肿瘤的生物标志物。
注：上述文献为领域内典型研究方向示例，实际引用时建议通过PubMed或Web of Science以“SEPT12 antibody”为关键词检索最新文献。
The SEPT12 antibody is a crucial tool for studying the SEPTIN12 (Septin 12) protein, a member of the septin family involved in cytoskeletal organization, cell division, and membrane dynamics. SEPT12 is particularly significant in male reproductive biology, where it plays essential roles in spermatogenesis, sperm head shaping, and tail formation. Mutations or dysregulation of SEPT12 have been linked to male infertility, often associated with abnormal sperm morphology, such as teratozoospermia. The antibody enables researchers to detect and quantify SEPT12 expression in tissues, particularly in the testis, and to investigate its localization and interactions during germ cell development.
Commercially available SEPT12 antibodies are typically developed in rabbits or mice using immunogenic peptides or recombinant protein fragments. They are validated for applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specificity is confirmed via knockout controls or siRNA-mediated knockdown. In research, the antibody aids in elucidating SEPT12's role in fertility disorders and its potential connection to broader cellular processes, such as cancer pathogenesis, where septins are implicated in tumorigenesis. Proper validation ensures reliability in distinguishing SEPT12 from other septin family members (e.g., SEPT4 or SEPT7), which share structural homology but have distinct functions. Overall, the SEPT12 antibody is indispensable for advancing studies in reproductive medicine and cellular biology.
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