| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RING finger protein 223, RNF223 |
| Entrez GeneID | 401934 |
| WB Predicted band size | 26.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RNF223 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-134 amino acids from the Central region of human RNF223. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是基于模拟生成的关于RNF223抗体的参考文献示例(实际文献可能需要通过学术数据库验证)：
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1. **文献名称**: *RNF223 Regulates Neuronal Development via Ubiquitination of β-Catenin*
**作者**: Li X, Wang Y, Zhang Q
**摘要**: 本研究利用RNF223特异性抗体，通过免疫共沉淀和Western blot技术，揭示了RNF223通过泛素化β-catenin调控Wnt信号通路，影响神经元分化和轴突生长的分子机制。
2. **文献名称**: *Characterization of a Novel RNF223 Antibody for Immunohistochemical Analysis in Breast Cancer*
**作者**: Chen H, et al.
**摘要**: 报道了一种高特异性RNF223多克隆抗体的开发，验证了其在乳腺癌组织中的表达模式，并发现RNF223低表达与患者预后不良相关，提示其潜在的肿瘤抑制功能。
3. **文献名称**: *RNF223-Mediated Ubiquitination in Cardiac Hypertrophy*
**作者**: Kim S, Park JH, Lee D
**摘要**: 通过RNF223抗体检测发现，其在心脏肥大小鼠模型中表达上调，进一步功能实验表明RNF223通过泛素化降解TAK1蛋白，调控MAPK信号通路参与病理过程。
4. **文献名称**: *RNF223 Antibody Validation for Flow Cytometry Applications*
**作者**: Gonzalez R, et al.
**摘要**: 验证了商业化的RNF223抗体在流式细胞术中的适用性，确认其在免疫细胞亚群(如T细胞和巨噬细胞)中的表达差异，为免疫调节研究提供工具支持。
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**说明**：以上文献为模拟生成，实际研究中RNF223的相关报道可能较少。建议通过 **PubMed**、**Google Scholar** 或 **Web of Science** 以关键词“RNF223 antibody”或“RNF223 function”检索最新文献。如需具体文献协助，可提供更多研究背景信息。
The RNF223 antibody is a research tool designed to detect and study the RING finger protein 223 (RNF223), a member of the RING finger family of E3 ubiquitin ligases. RNF223 contains a characteristic RING domain, which facilitates ubiquitination—a critical post-translational modification involved in protein degradation, signaling, and cellular regulation. While the precise biological functions of RNF223 remain under investigation, it is hypothesized to participate in ubiquitin-mediated pathways influencing cell proliferation, apoptosis, or stress responses. Its expression has been observed in various tissues, suggesting roles in both physiological and pathological processes.
Antibodies targeting RNF223 are typically developed in model organisms (e.g., rabbits or mice) using immunogenic peptides or recombinant protein fragments. These antibodies enable researchers to investigate RNF223’s expression levels, subcellular localization, and interactions via techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Validation often includes specificity tests (e.g., knockout/knockdown controls) to ensure minimal cross-reactivity with homologous proteins. Interest in RNF223 has grown in contexts such as cancer, neurodegenerative disorders, and metabolic diseases, where ubiquitination dysregulation is implicated. However, comprehensive mechanistic insights remain limited, highlighting the need for further studies using tools like RNF223 antibodies to unravel its molecular and therapeutic relevance.
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