| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein MIXL1, Homeodomain protein MIX, hMix, MIX1 homeobox-like protein 1, Mix1 homeobox-like protein, MIXL1, MIXL |
| Entrez GeneID | 83881 |
| WB Predicted band size | 24.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This MIXL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids from the Central region of human MIXL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MIXL1抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"MIXL1 regulates axial mesendoderm morphogenesis and patterning in human embryonic development"*
**作者**：Davis, R.P., et al.
**摘要**：研究通过MIXL1抗体进行免疫荧光染色，揭示了MIXL1在人类胚胎干细胞向中内胚层分化中的关键作用，证明其参与调控早期体轴形成和细胞迁移。
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2. **文献名称**：*"A functional role for MIXL1 in metastatic colorectal cancer progression"*
**作者**：Smith, J.G., et al.
**摘要**：利用MIXL1抗体进行免疫组化分析，发现MIXL1在结直肠癌转移组织中高表达，并与其通过Wnt/β-catenin通路促进肿瘤侵袭性的机制相关。
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3. **文献名称**：*"Optimization of MIXL1 antibody for flow cytometry in hematopoietic differentiation studies"*
**作者**：Chen, L., et al.
**摘要**：本文优化了MIXL1抗体在流式细胞术中的应用条件，验证其在造血祖细胞分化模型中对MIXL1蛋白动态表达的检测可靠性。
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*注：以上文献信息为示例性质，实际引用时需核实具体论文细节及数据库收录情况。如需进一步检索，建议通过PubMed或Google Scholar以关键词“MIXL1 antibody”结合研究领域(如发育生物学、癌症)筛选文献。*
The MIXL1 antibody is a tool used to detect the Mix paired-like homeobox protein 1 (MIXL1), a transcription factor encoded by the *MIXL1* gene. MIXL1 belongs to the homeobox family of proteins, which play critical roles in embryonic development, particularly in mesoderm and endoderm formation. It regulates gene expression during gastrulation and early organogenesis, interacting with signaling pathways such as Wnt and Nodal to establish body axis patterning and cell fate specification.
In research, MIXL1 antibodies are widely employed in developmental biology and stem cell studies. They help identify MIXL1-expressing cells, such as mesodermal progenitors, in differentiating embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs). These antibodies are also used in techniques like Western blotting, immunofluorescence, and flow cytometry to study lineage commitment and early differentiation processes.
Dysregulation of MIXL1 has been implicated in certain cancers, including colorectal and hematologic malignancies, where it may influence tumor cell proliferation or metastasis. The antibody thus serves as a biomarker in cancer research. Most MIXL1 antibodies target conserved regions of the protein, often recognizing human, mouse, or rat homologs. Validation data (e.g., knockout controls) are essential to confirm specificity, as cross-reactivity with other homeobox proteins can occur. Overall, MIXL1 antibodies are vital for unraveling developmental mechanisms and disease pathways linked to this key regulatory protein.
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