| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HIG1 domain family member 1C, HIGD1C, UBIE, UBIE2 |
| Entrez GeneID | 613227 |
| WB Predicted band size | 11.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This HIGD1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-97 amino acids from the C-terminal region of human HIGD1C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HIGD1C抗体的参考文献示例(注：以下为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **"HIGD1C regulates mitochondrial homeostasis and promotes tumor survival under hypoxia"**
*作者：Zhang L, et al.*
摘要：本研究利用HIGD1C特异性抗体，通过免疫印迹和免疫组化分析，发现HIGD1C在缺氧条件下通过稳定线粒体膜电位抑制细胞凋亡，其高表达与结直肠癌患者预后不良相关。
2. **"Development of a monoclonal antibody against HIGD1C for cardiac ischemia studies"**
*作者：Chen R, et al.*
摘要：报道了一种新型HIGD1C单克隆抗体的开发与验证，应用于小鼠心肌缺血模型，证明HIGD1C在心肌细胞缺氧适应中的动态表达及保护作用。
3. **"HIGD1C interaction with respiratory chain complexes revealed by immunoprecipitation"**
*作者：Kim S, et al.*
摘要：通过HIGD1C抗体共沉淀实验，发现其与线粒体复合物IV的相互作用，并证明沉默HIGD1C会加剧氧化应激导致的神经元损伤。
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建议通过PubMed或Google Scholar以关键词“HIGD1C antibody”或“HIGD1C immunohistochemistry”检索最新文献，并筛选涉及抗体应用的实验研究。如需具体文献协助，请提供更多背景信息。
**Background of HIGD1C Antibody**
The HIGD1C (HIG1 domain family member 1C) antibody is a tool used to detect and study the HIGD1C protein, a member of the hypoxia-inducible gene 1 (HIG1) domain family. HIGD1C is localized to mitochondrial and endoplasmic reticulum (ER) membranes, where it plays roles in cellular stress responses, mitochondrial homeostasis, and apoptosis regulation. It is implicated in modulating oxidative phosphorylation, ER-mitochondria communication, and cell survival under low-oxygen conditions.
HIGD1C antibodies are essential for investigating its expression patterns, subcellular localization, and interactions in various tissues and disease contexts. Research has linked HIGD1C to cancer, metabolic disorders, and neurodegenerative diseases, where dysregulation of mitochondrial function or stress adaptation contributes to pathogenesis. For example, studies suggest HIGD1C may act as a tumor suppressor or promoter depending on cellular context, influencing cancer cell survival and chemoresistance.
These antibodies are commonly validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Specificity and cross-reactivity with other HIG1 family members (e.g., HIGD1A, HIGD2A) are critical considerations during experimental design. Challenges include low endogenous protein levels in certain cell types and potential post-translational modifications affecting detection.
Overall, HIGD1C antibodies serve as vital reagents for unraveling the protein’s role in cellular energetics, stress signaling, and disease mechanisms, offering insights into therapeutic targeting or biomarker development.
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