| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein CASC5, Cancer susceptibility candidate gene 5 protein homolog, Kinetochore-null protein 1, Casc5, Knl1 |
| WB Predicted band size | 179.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Casc5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1557-1583 amino acids from the C-terminal region of mouse Casc5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于小鼠CASC5抗体的3篇参考文献示例(注：以下文献信息为模拟示例，实际文献需通过数据库检索确认)：
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1. **文献名称**: *"Characterization of CASC5/KNL1 Antibody for Mitotic Studies in Mouse Embryonic Fibroblasts"*
**作者**: Smith J, et al.
**摘要**: 本研究验证了一种针对小鼠CASC5蛋白的单克隆抗体，通过免疫印迹和免疫荧光证实其特异性。实验表明该抗体能有效识别有丝分裂过程中CASC5在动粒上的定位，为研究染色体分离机制提供了工具。
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2. **文献名称**: *"Development of a Polyclonal Antibody Against Murine CASC5 and Its Application in Meiotic Analysis"*
**作者**: Lee H, et al.
**摘要**: 文章报道了一种兔源多克隆抗体的制备，该抗体靶向小鼠CASC5的N端结构域。通过免疫沉淀和共聚焦显微镜验证，证明其在减数分裂研究中可有效检测CASC5与MIS12复合体的相互作用。
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3. **文献名称**: *"CASC5 Depletion Disrupts Kinetochore Assembly: Insights from Antibody-Based Knockdown in Mouse Oocytes"*
**作者**: Gonzalez R, et al.
**摘要**: 利用针对小鼠CASC5的抗体进行功能阻断实验，发现其缺失导致动粒组装异常和染色体排列错误。研究强调了CASC5在卵母细胞分裂中的关键作用，并提供了抗体用于体内功能验证的方法。
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如需具体文献，建议在PubMed或Google Scholar中检索关键词：“CASC5 antibody mouse”“KNL1 antibody murine”或结合研究领域如“kinetochore antibody validation”。
**Background of Mouse CASC5 Antibody**
The CASC5 (Cancer Susceptibility Candidate 5) protein, also known as KNL1. is a critical component in mitotic regulation, particularly in kinetochore assembly and spindle assembly checkpoint (SAC) signaling during cell division. It acts as a scaffold for recruiting other SAC proteins, such as BUB1 and BUBR1. ensuring proper chromosome segregation and genomic stability. Dysregulation of CASC5 is linked to chromosomal instability and cancer progression.
Mouse CASC5 antibodies are specifically designed to detect the murine homolog of this protein. These antibodies are typically generated using immunogenic peptides or recombinant fragments of the mouse CASC5 protein, often in hosts like rabbits or goats. They are widely employed in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to study CASC5 expression, localization, and interactions in murine cell lines or tissues.
Research utilizing Mouse CASC5 antibodies has advanced understanding of its role in mitosis, SAC signaling, and its implications in diseases like cancer. Validated antibodies are essential for distinguishing CASC5 isoforms and assessing post-translational modifications. Such tools are pivotal in exploring therapeutic strategies targeting mitotic errors in oncology or developmental disorders. Specificity and cross-reactivity with human or other species' CASC5 should be confirmed experimentally.
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