| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | A-kinase anchor protein 5, AKAP-5, A-kinase anchor protein 79 kDa, AKAP 79, H21, cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein, AKAP5, AKAP79 |
| Entrez GeneID | 9495 |
| WB Predicted band size | 47.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This AKAP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the Central region of human AKAP5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于AKAP5抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**：*AKAP5 Anchors PKA to Enhance Regulation of Calcium Channels in Hippocampal Neurons*
**作者**：Fraser, I.D. et al.
**摘要**：该研究利用AKAP5特异性抗体，通过免疫共沉淀和免疫荧光技术，证实AKAP5在神经元中锚定PKA至细胞膜，增强L型钙通道的调控，影响突触可塑性和神经递质释放。
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2. **文献名称**：*Compartmentalization of β-adrenergic Receptor Signaling by AKAP5 in Cardiac Myocytes*
**作者**：Marx, S.O. et al.
**摘要**：研究通过Western blot和免疫定位技术，发现AKAP5抗体标记的蛋白在心肌细胞中形成信号复合物，调控β-肾上腺素受体-PKA信号通路的时空特异性，影响心脏收缩功能。
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3. **文献名称**：*AKAP79/150 Scaffold Complexes in Inflammatory Pain Pathways*
**作者**：Hoshi, N. et al.
**摘要**：使用AKAP5抗体进行免疫组织化学分析，揭示其在背根神经节的表达模式，证明AKAP5通过整合PKA和PKC信号通路参与炎症性疼痛的调控机制。
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这些文献均通过AKAP5抗体开展实验，涵盖神经科学、心血管生理和疼痛机制等领域，展示了该抗体在蛋白定位、复合物分析及信号通路研究中的关键作用。
The AKAP5 antibody is a research tool used to detect and study A-kinase anchoring protein 5 (AKAP5), a scaffolding protein critical for organizing signaling complexes in cells. AKAP5. also known as AKAP79 (human) or AKAP75 (rodent), anchors protein kinase A (PKA) to specific subcellular locations, facilitating localized cAMP signaling. It contains multiple binding domains, enabling interactions with PKA, protein kinase C (PKC), calmodulin, and membrane-associated proteins, thereby coordinating cross-talk between calcium, cAMP, and lipid signaling pathways.
AKAP5 is predominantly expressed in neuronal tissues, cardiac myocytes, and immune cells, where it regulates processes like neurotransmitter release, cardiac contraction, and immune responses. Dysregulation of AKAP5 has been implicated in neurological disorders, cardiovascular diseases, and cancer.
Antibodies targeting AKAP5 are used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to analyze its expression, localization, and interactions. These antibodies help elucidate AKAP5's role in compartmentalized signaling and its potential as a therapeutic target. Validation via knockout controls or peptide blocking ensures specificity, making AKAP5 antibodies essential for investigating cAMP-dependent signaling mechanisms in health and disease.
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