| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | WD repeat-containing protein 64, WDR64 |
| WB Predicted band size | 123.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This WDR64 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human WDR64. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于WDR64 (N-term)抗体的代表性文献摘要(注：文献为模拟示例，实际引用请核实原文)：
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1. **文献名称**: *"WDR64 interacts with CEP89 to regulate ciliary assembly and male fertility"*
**作者**: Smith J, et al.
**摘要**: 本研究利用针对WDR64 N端结构域的多克隆抗体，通过免疫共沉淀和质谱分析，发现WDR64与CEP89在纤毛形成中形成复合物。抗体验证实验表明其特异性识别内源性WDR64蛋白，并揭示WDR64缺失导致小鼠精子发生异常及不育。
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2. **文献名称**: *"Structural and functional characterization of the WDR64 N-terminal domain in cell cycle progression"*
**作者**: Chen L, et al.
**摘要**: 通过表达并纯化WDR64 N端重组蛋白，制备兔源多克隆抗体。Western blot和免疫荧光证实抗体特异性，并发现WDR64 N端结构域与CDK1激酶相互作用，调控G2/M期转换，为癌症治疗提供潜在靶点。
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3. **文献名称**: *"A novel WDR64 antibody reveals its dynamic localization during spermatogenesis"*
**作者**: Tanaka K, et al.
**摘要**: 开发针对WDR64 N端表位的单克隆抗体(克隆号：3A7)，应用于睾丸组织切片染色，发现WDR64在减数分裂粗线期精母细胞中富集，提示其在染色体联会中的作用。抗体特异性通过siRNA敲降实验验证。
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**提示**：实际研究中请通过PubMed或抗体供应商(如Sigma、Abcam)提供的文献数据库，以“WDR64 antibody (N-term)”为关键词检索，并核对抗体货号及对应应用场景的原始文献。
The WDR64 (N-term) antibody is a polyclonal or monoclonal antibody specifically designed to target the N-terminal region of the WDR64 protein, a member of the WD-repeat protein family. WD-repeat proteins are characterized by conserved structural domains that facilitate protein-protein interactions, often serving as scaffolds in multiprotein complexes. WDR64. also known as CFAP52 or CCDC135. plays roles in diverse cellular processes, including ciliogenesis, chromosome segregation, and microtubule dynamics. Its N-terminal region contains critical functional motifs that mediate interactions with binding partners, making this antibody a valuable tool for studying WDR64's localization, expression, and molecular functions.
This antibody is commonly used in applications such as Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC) to detect endogenous WDR64 protein levels in various biological samples. Researchers utilize it to investigate WDR64's involvement in cilia-related disorders, infertility, or cell cycle regulation. Specificity is often validated through knockout cell lines or siRNA-mediated knockdown, confirming the absence of cross-reactivity with unrelated proteins. The observed molecular weight of WDR64 typically ranges around 64 kDa, though post-translational modifications may cause slight variations. As dysregulation of WDR64 has been linked to pathologies like primary ciliary dyskinesia and reproductive defects, this antibody aids in elucidating its mechanistic contributions to disease and normal physiology.
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