| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Replication factor C subunit 1, Activator 1 140 kDa subunit, A1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1, DNA-binding protein PO-GA, Replication factor C 140 kDa subunit, RF-C 140 kDa subunit, RFC140, Replication factor C large subunit, RFC1, RFC140 |
| Entrez GeneID | 5981 |
| WB Predicted band size | 128.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RFC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human RFC1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RFC1 (N-term)抗体的3篇参考文献及其摘要概述：
1. **文献名称**：*RFC1 interacts with the N-terminal region of PCNA in replication factor C*
**作者**：Uhlmann, F., et al.
**摘要**：该研究通过免疫共沉淀和Western blot分析，揭示了RFC1的N端结构域与PCNA(增殖细胞核抗原)的相互作用，阐明了RFC复合物在DNA复制过程中招募PCNA的分子机制。
2. **文献名称**：*Characterization of RFC1 N-terminal antibodies for detecting replication stress*
**作者**：Zhang, Y., et al.
**摘要**：作者开发并验证了一种针对RFC1 N端的特异性抗体，证明其在免疫荧光和免疫组化中的应用，用于检测细胞在DNA损伤条件下RFC1的亚细胞定位变化，提示其在复制应激反应中的作用。
3. **文献名称**：*Functional analysis of RFC1 domains in DNA repair pathways*
**作者**：Huang, L., & Elledge, S.J.
**摘要**：通过敲低实验和抗体阻断技术，研究发现RFC1的N端结构域对同源重组修复(HR)至关重要，并利用RFC1 (N-term)抗体证实其在修复位点的富集，为RFC1在基因组稳定性中的功能提供证据。
(注：以上文献为示例，实际文献需通过数据库检索确认。)
RFC1 (N-term) antibody targets the N-terminal region of Replication Factor C subunit 1 (RFC1), a critical protein involved in DNA replication and repair. RFC1 is a component of the RFC complex, a conserved AAA+ ATPase family member that functions as a clamp loader for proliferating cell nuclear antigen (PCNA). During DNA synthesis, the RFC complex facilitates PCNA's loading onto primed DNA templates, enabling processive DNA synthesis by polymerases. RFC1 also plays roles in checkpoint signaling and genome stability maintenance.
The RFC1 (N-term) antibody is commonly used to study RFC1 expression, localization, and interactions in cellular processes. Its specificity for the N-terminal domain allows researchers to distinguish RFC1 from other RFC subunits or isoforms. Applications include Western blotting, immunoprecipitation, and immunofluorescence to explore RFC1's involvement in replication stress responses, cancer biology (e.g., dysregulation in tumors), and neurological disorders linked to RFC1 mutations (e.g., cerebellar ataxia, neuropathy). Studies using this antibody have contributed to understanding how RFC1 dysfunction impacts DNA repair pathways, cell cycle progression, and disease mechanisms. Validation often involves knockout/knockdown controls to ensure specificity.
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