| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ubiquitin-conjugating enzyme E2 D4, HBUCE1, Ubiquitin carrier protein D4, Ubiquitin-protein ligase D4, UBE2D4, UBCH5D |
| Entrez GeneID | 51619 |
| WB Predicted band size | 16.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This UBE2D4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human UBE2D4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于UBE2D4抗体的示例参考文献(注：以下内容为模拟示例，实际文献可能需要通过数据库进一步检索验证)：
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1. **文献名称**: *"Characterization of UBE2D4-Specific Antibodies for Detecting Ubiquitination in Breast Cancer Models"*
**作者**: Smith A, et al.
**摘要**: 本研究开发并验证了一种高特异性UBE2D4抗体，用于检测乳腺癌细胞系中UBE2D4介导的泛素化修饰。通过免疫印迹和免疫沉淀实验，证实该抗体可有效识别内源性UBE2D4蛋白，并揭示其在HER2信号通路中的调控作用。
2. **文献名称**: *"UBE2D4 Antibody Application in Neurodegenerative Disease Proteomics"*
**作者**: Lee JH, et al.
**摘要**: 文章利用UBE2D4抗体对阿尔茨海默病模型小鼠的脑组织进行蛋白质组学分析，发现UBE2D4与tau蛋白异常泛素化显著相关，提示其可能参与神经退行性病变的蛋白质稳态失调机制。
3. **文献名称**: *"A Novel Monoclonal Antibody Targeting UBE2D4 Reveals Its Subcellular Localization and Functional Role in DNA Repair"*
**作者**: García-Ruiz C, et al.
**摘要**: 通过开发单克隆UBE2D4抗体，研究揭示了UBE2D4在细胞核内的定位及其与BRCA1蛋白的相互作用，证明其在DNA损伤修复过程中通过泛素化调控修复因子的稳定性。
4. **文献名称**: *"Validation of Commercial UBE2D4 Antibodies for Immunohistochemical Staining in Human Tissues"*
**作者**: Wang X, et al.
**摘要**: 对比分析了多种市售UBE2D4抗体的特异性，筛选出适用于石蜡包埋组织切片的抗体，并发现UBE2D4在结肠癌组织中的高表达与患者预后不良相关。
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**建议**：实际研究中，建议通过PubMed、Web of Science等平台以“UBE2D4 antibody”为关键词检索最新文献，并优先选择经过实验验证(如KO验证、应用数据)的研究。部分研究可能未直接以UBE2D4抗体为核心，但可能在其方法学部分提及。
The UBE2D4 antibody is a tool used to detect and study the UBE2D4 protein, a member of the ubiquitin-conjugating enzyme (E2) family. UBE2D4. also known as UBCH5D, plays a role in the ubiquitin-proteasome system, a critical pathway for protein degradation and regulation. As an E2 enzyme, UBE2D4 collaborates with E3 ubiquitin ligases to transfer ubiquitin molecules to target proteins, marking them for proteasomal degradation or modulating their function in processes like cell cycle regulation, DNA repair, and signal transduction. Compared to other UBE2D family members (UBE2D1-D3), UBE2D4 is less characterized, though it shares structural homology and overlapping roles in ubiquitination.
Antibodies against UBE2D4 are essential for investigating its expression, localization, and interactions in cellular contexts. They are utilized in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to explore its biological functions and dysregulation in diseases. Specificity and sensitivity are critical, as cross-reactivity with other UBE2D isoforms is a potential challenge. Validation often involves knockout controls or mass spectrometry to confirm target recognition. Research on UBE2D4 is ongoing, with studies suggesting potential links to cancer, neurodegenerative disorders, and immune responses. Its antibody thus serves as a key reagent for advancing understanding of ubiquitination dynamics and therapeutic targeting of this pathway.
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