| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | T-box transcription factor TBX4, T-box protein 4, TBX4 |
| Entrez GeneID | 9496 |
| WB Predicted band size | 60.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This TBX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-129 amino acids from the N-terminal region of human TBX4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TBX4(N-term)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
1. **文献名称**：*TBX4 mutations cause small patella syndrome*
**作者**：Bongers EM et al.
**摘要**：该研究首次报道了TBX4基因突变与小儿髌骨发育不全综合征的关联。文中提到使用针对TBX4 N端区域的抗体进行Western blot和免疫组化实验，验证了突变导致的蛋白截断对TBX4功能的影响，并证实该抗体在患者样本中的特异性结合。
2. **文献名称**：*Limb and lung defects in Tbx4 gene-targeted mice*
**作者**：Naiche LA, Papaioannou VE
**摘要**：研究通过基因敲除小鼠模型探讨TBX4在肺部和肢体发育中的作用。作者利用N端特异性TBX4抗体进行免疫荧光染色，显示TBX4在胚胎肺间质细胞和后肢芽中的表达模式，并验证抗体在组织定位分析中的可靠性。
3. **文献名称**：*Characterization of a polyclonal antibody against the N-terminal region of TBX4 for developmental studies*
**作者**：Sakabe M et al.
**摘要**：该文献详细描述了一种针对TBX4蛋白N端区域的多克隆抗体的开发与验证。通过肽段免疫制备抗体后，研究团队利用免疫沉淀(IP)和免疫印迹(WB)证明其特异性，并应用于鸡胚模型，揭示TBX4在早期肺分支形态发生中的表达动态。
*注：若需具体文献来源(如期刊、年份)，建议通过PubMed或Google Scholar输入上述标题或作者名进一步检索。部分研究可能涉及抗体应用但不以抗体开发为主，需结合实验方法部分筛选。*
The TBX4 (N-term) antibody is a specialized tool designed to detect the N-terminal region of the T-box transcription factor 4 (TBX4), a critical regulator of embryonic development. TBX4. part of the evolutionarily conserved T-box gene family, plays essential roles in organogenesis, particularly in lung branching morphogenesis, posterior limb development, and urogenital system formation. It functions by binding to DNA through its conserved T-box domain, regulating target genes involved in cell differentiation and tissue patterning. Mutations in TBX4 are linked to congenital disorders such as small patella syndrome and pulmonary arterial hypertension.
The antibody is generated using immunogens corresponding to the N-terminal region of human TBX4. enabling specific recognition of full-length TBX4 or isoforms retaining this domain. It is commonly validated in applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), often with supporting knockout/knockdown controls to confirm specificity. Researchers use this antibody to study TBX4's role in developmental pathways (e.g., FGF, BMP signaling) and diseases, including cancer, where TBX4 may act as a tumor suppressor or oncogene depending on context. Its specificity for TBX4 over homologous T-box proteins (e.g., TBX2. TBX5) makes it valuable for dissecting precise molecular mechanisms in both normal and pathological states.
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