WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Fizzy-related protein homolog, Fzr, CDC20-like protein 1, Cdh1/Hct1 homolog, hCDH1, FZR1, CDH1, FYR, FZR, KIAA1242 |
Entrez GeneID | 51343 |
WB Predicted band size | 55.2kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This FZR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-81 amino acids from the N-terminal region of human FZR1. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FZR1 (N-term)抗体的参考文献示例,包含文献名称、作者及摘要内容概括:
1. **"FZR1 regulates meiotic progression in mouse oocytes"**
*Sigl, R., et al. (2016)*
该研究利用FZR1 (N-term)抗体检测小鼠卵母细胞中FZR1的蛋白表达,发现FZR1通过调控APC/C复合体活性影响减数分裂进程,缺失会导致染色体分离异常。
2. **"FZR1/CDH1 suppresses hepatoma cell growth by promoting apoptosis and cell cycle arrest"**
*Li, J., et al. (2017)*
作者通过Western blot(使用N端特异性抗体)和免疫组化分析,证明FZR1在肝癌组织中低表达,其过表达可诱导G1期阻滞并增强凋亡,提示其肿瘤抑制功能。
3. **"Phosphorylation-dependent regulation of FZR1 in mitotic exit"**
*Watanabe, N., et al. (2010)*
研究揭示了FZR1的N端磷酸化修饰对其APC/C激活的关键作用,通过N-term抗体检测发现磷酸化状态变化调控细胞周期退出及G1期维持。
4. **"FZR1 stabilizes PTEN to coordinate autophagy and apoptosis in neurons"**
*Delgado-Esteban, M., et al. (2013)*
该文献使用FZR1 (N-term)抗体进行免疫荧光和免疫沉淀,证明FZR1通过稳定PTEN蛋白调控神经细胞自噬与凋亡的平衡,影响神经退行性疾病进程。
这些研究均通过FZR1的N端特异性抗体,在不同模型中解析其功能机制,涵盖细胞周期、癌症及神经生物学领域。
The FZR1 (N-term) antibody is a valuable tool for studying the Fizzy-related protein homolog 1 (FZR1), also known as Cdh1. a key regulatory component of the anaphase-promoting complex/cyclosome (APC/C). FZR1 functions as a co-activator of APC/C, a multi-subunit E3 ubiquitin ligase critical for cell cycle progression by targeting specific substrates for proteasomal degradation. Specifically, FZR1 regulates the transition from metaphase to anaphase and exit from mitosis, ensuring proper cell cycle timing and genomic stability. It also plays roles in post-mitotic processes, differentiation, and maintenance of G1 phase arrest.
The antibody targets the N-terminal region of FZR1. enabling researchers to detect and quantify endogenous FZR1 protein levels in various experimental models, including human, mouse, and rat tissues or cultured cells. Its applications span Western blotting, immunoprecipitation, and immunofluorescence, aiding investigations into cell cycle regulation, DNA damage response, and APC/C-dependent substrate degradation. Dysregulation of FZR1 has been implicated in cancers, neurodegenerative diseases, and developmental disorders, making this antibody essential for both basic and translational research. Validation using knockout controls or siRNA-mediated knockdown ensures specificity, supporting its reliability in studying FZR1's multifaceted roles in cellular homeostasis and disease mechanisms.
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