| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RUN domain-containing protein 3A, Rap2-interacting protein 8, RPIP-8, RUNDC3A, RAP2IP, RPIP8 |
| Entrez GeneID | 10900 |
| WB Predicted band size | 49.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RUNDC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-157 amino acids from the Central region of human RUNDC3A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RUNDC3A抗体的模拟参考文献示例(实际文献需通过学术数据库验证)：
1. **文献名称**：RUNDC3A在乳腺癌中的抑癌作用及抗体开发
**作者**：Chen L, et al.
**摘要**：本研究通过构建RUNDC3A特异性抗体，验证其在乳腺癌细胞系中的表达下调，并发现其通过调控PI3K/AKT通路抑制肿瘤侵袭。
2. **文献名称**：RUNDC3A抗体在神经突触可塑性研究中的应用
**作者**：Wang Y, et al.
**摘要**：利用RUNDC3A多克隆抗体进行免疫荧光染色，揭示该蛋白在海马神经元突触形成中的定位，提示其参与学习记忆相关通路。
3. **文献名称**：RUNDC3A与自噬相关蛋白相互作用的鉴定
**作者**：Kim S, et al.
**摘要**：通过免疫共沉淀(Co-IP)结合RUNDC3A抗体，发现其与LC3蛋白存在相互作用，可能参与细胞自噬调控过程。
4. **文献名称**：结直肠癌中RUNDC3A表达缺失的临床意义
**作者**：Zhang R, et al.
**摘要**：采用RUNDC3A单克隆抗体对组织微阵列进行免疫组化分析，发现其低表达与患者预后不良显著相关。
注：以上内容为模拟生成，建议通过PubMed、Google Scholar等平台检索真实文献。
The RUNDC3A (RUN Domain Containing 3A) antibody is a tool used to detect and study the RUNDC3A protein, encoded by the RUNDC3A gene. This gene is evolutionarily conserved and widely expressed in tissues, particularly in the brain and testis. The RUNDC3A protein contains a RUN domain, a structural motif implicated in protein-protein interactions and cellular signaling pathways. While its precise biological role remains under investigation, RUNDC3A is suggested to participate in autophagy regulation, neuronal development, and cancer progression. Studies link it to tumor suppression in glioblastoma and hepatocellular carcinoma, though conflicting reports associate it with pro-metastatic functions in other cancers, highlighting context-dependent roles.
RUNDC3A antibodies, typically polyclonal or monoclonal, are generated using immunogenic peptide sequences from conserved regions of the protein. These antibodies enable researchers to explore RUNDC3A's expression patterns, subcellular localization (e.g., cytoplasmic or membrane-associated), and interactions via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They are critical for elucidating RUNDC3A's involvement in diseases, including neurodegenerative disorders and cancer. However, challenges persist in antibody specificity due to potential cross-reactivity with homologous proteins or splice variants. Recent studies also investigate its post-translational modifications and potential as a therapeutic target. Continued validation of RUNDC3A antibodies remains essential for resolving its multifaceted roles in cellular physiology and pathology.
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