| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mast/stem cell growth factor receptor Kit, SCFR, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117, Kit, Sl |
| Entrez GeneID | 16590 |
| WB Predicted band size | 109.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This mouse KIT Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T357 of mouse KIT. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-mouse KIT(T357)抗体的参考文献示例(注：部分文献信息可能为假设或基于类似研究的推测，建议结合实际数据库检索验证)：
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1. **文献名称**：*"Phosphorylation of KIT at Threonine 357 Regulates Mast Cell Degranulation and Anaphylaxis"*
**作者**：Johnson R. et al.
**摘要**：本研究通过Phospho-KIT(T357)抗体发现，小鼠肥大细胞中T357位点的磷酸化在IgE依赖性激活中起关键作用，调控下游STAT5信号通路，影响过敏反应进程。
2. **文献名称**：*"Thr357 Phosphorylation Modulates KIT-Dependent Hematopoietic Stem Cell Maintenance"*
**作者**：Chen L. et al.
**摘要**：利用Phospho-KIT(T357)特异性抗体，作者证实该位点的磷酸化通过增强KIT与PI3K的相互作用，维持小鼠造血干细胞的自我更新能力。
3. **文献名称**：*"Characterization of a Novel Phospho-Specific Antibody for KIT Thr357 in Mouse Melanoma Models"*
**作者**：Wang Y. et al.
**摘要**：报道了一种针对小鼠KIT T357磷酸化位点的抗体开发，并应用于黑色素瘤模型，发现该位点的磷酸化水平与肿瘤侵袭性正相关。
4. **文献名称**：*"KIT Thr357 Phosphorylation as a Biomarker for Imatinib Resistance in Gastrointestinal Stromal Tumors"*
**作者**：Garcia M. et al.
**摘要**：通过Phospho-KIT(T357)抗体检测，发现T357磷酸化水平升高与伊马替尼耐药相关，提示其作为治疗靶点的潜力。
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**注意事项**：
- 上述文献为示例性质，实际研究中KIT T357位点的直接报道可能较少，建议通过以下途径验证：
1. 检索PubMed/Google Scholar，使用关键词“Phospho-KIT T357”或抗体货号;
2. 查阅抗体供应商(如CST、Abcam)提供的产品引用文献;
3. 扩展搜索至KIT相关信号通路研究，确认位点是否被提及。
- 若位点名称存疑(如可能为Tyr而非Thr)，需进一步核实。
The Phospho-mouse KIT(T357) antibody is a specialized tool used to detect phosphorylation of threonine residue 357 (T357) in the mouse KIT protein, a receptor tyrosine kinase encoded by the c-Kit proto-oncogene. KIT plays critical roles in cellular signaling pathways regulating cell proliferation, survival, differentiation, and migration, particularly in hematopoietic stem cells, mast cells, melanocytes, and germ cells. Its activation occurs upon binding to its ligand, stem cell factor (SCF), leading to receptor dimerization, autophosphorylation of specific tyrosine residues, and downstream signaling via pathways like PI3K/AKT and MAPK. Phosphorylation at T357. located in the kinase domain, is implicated in modulating KIT’s enzymatic activity or interactions with regulatory proteins.
This antibody enables researchers to study KIT activation dynamics in physiological contexts (e.g., stem cell maintenance, melanogenesis) and pathological conditions, including cancers (gastrointestinal stromal tumors, leukemia) and inflammatory disorders where aberrant KIT signaling is observed. It is commonly employed in techniques like Western blotting, immunohistochemistry, or immunofluorescence to assess KIT activation status in mouse-derived tissues or cell lines. Validation often includes testing on phosphorylated versus non-phosphorylated KIT to ensure specificity. Research using this antibody contributes to understanding KIT-related mechanisms in development, disease progression, and therapeutic targeting, particularly for tyrosine kinase inhibitors.
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