| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB |
| Entrez GeneID | 3551 |
| WB Predicted band size | 86.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S697 of human IKKB. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-IKKβ(S697)抗体的3篇代表性文献示例，内容基于相关领域研究的常见方向整合而成：
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1. **文献名称**：*Phosphorylation of IKKβ at serine 697 regulates NF-κB signaling by promoting nuclear translocation*
**作者**：Li, X. et al.
**摘要**：该研究阐明了IKKβ在S697位点的磷酸化通过促进其核转位增强NF-κB信号活性，使用Phospho-IKKβ(S697)抗体验证了DNA损伤后该位点的修饰，并揭示其与炎症因子释放的相关性。
2. **文献名称**：*A novel role of IKKβ C-terminal phosphorylation in TLR4-mediated macrophage activation*
**作者**：Chen, Z. et al.
**摘要**：作者利用Phospho-IKKβ(S697)特异性抗体，证明TLR4激活后诱导IKKβ的S697磷酸化，进而调控巨噬细胞中促炎基因(如TNF-α、IL-6)的表达，提示该位点可能成为炎症性疾病治疗的靶点。
3. **文献名称**：*Kinase-specific regulation of IKKβ phosphorylation at Ser697 by TBK1 in antiviral immunity*
**作者**：Wang, Y. et al.
**摘要**：研究发现病毒感染触发TBK1对IKKβ的S697位点磷酸化，通过抗体检测证实其磷酸化水平与干扰素通路激活相关，揭示了IKKβ非经典磷酸化在抗病毒免疫中的新机制。
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**说明**：以上文献为示例性质，实际研究中需根据具体抗体品牌(如CST、Abcam)提供的引用文献或通过PubMed等数据库检索关键词(如"IKKβ Ser697 phosphorylation")获取真实数据。建议结合实验背景补充最新研究。
The Phospho-IKKβ (S697) antibody is a specialized tool used to detect the activated form of IKKβ (Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Beta), a key regulatory kinase in the NF-κB signaling pathway. IKKβ, part of the IKK complex (IKKα, IKKβ, and NEMO/IKKγ), plays a central role in phosphorylating the inhibitor of NF-κB (IκB), leading to its degradation and subsequent activation of NF-κB transcription factors. This pathway is critical for immune responses, inflammation, and cell survival.
Phosphorylation at serine 697 (S697) is associated with IKKβ activation. While canonical activation involves phosphorylation at serine 177 and serine 181 in the activation loop, S697 phosphorylation has been reported in specific contexts, such as DNA damage responses or alternative signaling pathways. Studies suggest that S697 phosphorylation may regulate IKKβ’s kinase activity, substrate specificity, or interactions with other signaling components.
The Phospho-IKKβ(S697) antibody enables researchers to study the dynamics of IKKβ activation under various stimuli (e.g., cytokines, pathogens, stress) and in diseases like cancer, autoimmune disorders, or chronic inflammation. It is commonly used in techniques such as Western blotting, immunofluorescence, or immunoprecipitation to assess pathway activation. Validation of this antibody typically includes testing in knockout models or phosphorylation-blocking assays to ensure specificity. Understanding IKKβ phosphorylation patterns aids in dissecting NF-κB regulation and developing targeted therapies for inflammation-related pathologies.
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