| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB |
| Entrez GeneID | 3551 |
| WB Predicted band size | 86.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S675 of human IKKB. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-IKKB(S675)抗体的3篇参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：*IKKβ phosphorylation at serine 675 regulates NF-κB activation in response to inflammatory stimuli*
**作者**：Li, J. et al.
**摘要**：该研究证实IKKβ的S675磷酸化是炎症信号(如TNF-α)激活NF-κB通路的关键步骤，通过Phospho-IKKB(S675)抗体验证了该位点的动态修饰，并揭示其与激酶活性正相关。
2. **文献名称**：*A novel auto-phosphorylation site of IKKβ (Ser675) modulates its kinase activity in cancer cells*
**作者**：Chen, X. et al.
**摘要**：利用Phospho-IKKB(S675)特异性抗体，作者发现S675位点在多种癌细胞中发生自磷酸化，促进IKK复合体聚集并增强下游靶标(如IκBα)的降解，推动肿瘤生长。
3. **文献名称**：*Validation of a phospho-specific antibody for IKKβ Ser675 and its application in studying metabolic disorders*
**作者**：Wang, Y. et al.
**摘要**：本文系统验证了Phospho-IKKB(S675)抗体的特异性(通过点突变实验)，并应用于肥胖小鼠模型，证明高脂饮食诱导的S675磷酸化与胰岛素抵抗相关。
(注：以上文献为示例，实际引用需查询真实数据库如PubMed或抗体供应商提供的参考文献。)
The Phospho-IKKβ (S675) antibody is a critical tool for studying the activation of the nuclear factor-kappa B (NF-κB) signaling pathway. IKKβ (Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Beta), a key component of the IKK complex, plays a central role in phosphorylating the inhibitor of NF-κB (IκB), leading to its degradation and subsequent NF-κB nuclear translocation. Phosphorylation at serine 675 (S675) is associated with IKKβ activation, often triggered by pro-inflammatory cytokines (e.g., TNF-α, IL-1β), pathogens, or stress signals. This post-translational modification enhances IKKβ kinase activity, amplifying downstream inflammatory, immune, and survival responses.
Researchers use the Phospho-IKKβ (S675) antibody to detect and quantify IKKβ activation in experimental models, including Western blotting, immunofluorescence, and immunohistochemistry. Its specificity for the phosphorylated form allows precise assessment of pathway dynamics in conditions like cancer, autoimmune disorders, and neurodegenerative diseases. The antibody is particularly valuable for evaluating drug efficacy in preclinical studies, such as testing IKKβ inhibitors or anti-inflammatory agents. Proper validation, including knockout controls and stimulation assays (e.g., using LPS or TNF-α), ensures accurate interpretation. Understanding IKKβ phosphorylation at S675 provides insights into cellular stress responses and therapeutic targeting of NF-κB-driven pathologies.
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