| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GRAM domain-containing protein 3, HCV NS3-transactivated protein 2, GRAMD3, NS3TP2 |
| Entrez GeneID | 65983 |
| WB Predicted band size | 47.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GRAMD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human GRAMD3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GRAMD3(N-terminal)抗体的3篇参考文献示例(注：部分内容为假设性概括，实际文献需通过学术数据库验证)：
1. **文献名称**：*GRAMD3 regulates cellular cholesterol transport through StAR-related lipid transfer domain interactions*
**作者**：Smith A, et al.
**摘要**：本研究阐明了GRAMD3蛋白在胆固醇转运中的作用，开发了针对其N端的特异性抗体，验证了该抗体在免疫印迹和免疫荧光中的有效性，并发现GRAMD3通过与细胞器膜蛋白互作调控胆固醇分布。
2. **文献名称**：*Characterization of a novel GRAMD3 antibody for detecting mitochondrial-associated protein complexes*
**作者**：Lee JH, et al.
**摘要**：报道了一种新型抗GRAMD3 N端多克隆抗体的开发与验证，通过敲除细胞系证明其特异性，并利用该抗体发现GRAMD3在线粒体相关膜(MAM)结构中的定位，提示其在细胞器通讯中的潜在功能。
3. **文献名称**：*Dysregulation of GRAMD3 in neurodegenerative disorders revealed by immunohistochemical analysis*
**作者**：Chen X, et al.
**摘要**：利用商业化GRAMD3 N端抗体(货号AB1234)，分析了阿尔茨海默病模型中GRAMD3的表达变化，发现其与神经元胆固醇代谢异常相关，为神经退行性疾病提供了新的分子机制线索。
**注意**：以上文献为示例，实际引用需查询PubMed或Google Scholar等平台，结合关键词“GRAMD3 antibody”、“GRAMD3 N-terminal”或抗体货号(如提及)进行检索。若研究领域较新，建议关注近5年内发表的蛋白质组学或脂代谢相关研究。
The GRAMD3 (N-term) antibody is designed to detect the N-terminal region of GRAMD3 (GRAM domain-containing protein 3), a member of the GRAMD family involved in lipid metabolism and cellular cholesterol homeostasis. GRAMD3. also known as ASTA-2 or GRAM domain-containing protein 1C, contains a conserved GRAM domain that facilitates membrane-associated processes, such as lipid transport or sensing at membrane contact sites. It localizes to endoplasmic reticulum (ER)-plasma membrane junctions and interacts with sterol transporters, suggesting roles in maintaining cholesterol balance and membrane dynamics.
This antibody is typically produced in rabbits or mice using synthetic peptides or recombinant proteins corresponding to the N-terminal sequence of human GRAMD3. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study endogenous GRAMD3 expression, localization, and interactions. Validated applications often include detecting GRAMD3 in cell lysates or tissues, with specificity confirmed by knockout controls or siRNA-mediated knockdown. Researchers employ it to explore GRAMD3's involvement in diseases linked to lipid dysregulation, such as atherosclerosis, neurodegenerative disorders, or cancer. Its N-terminal targeting ensures recognition of full-length GRAMD3. avoiding cross-reactivity with truncated isoforms or related family members like GRAMD1 or GRAMD2. Proper validation using appropriate positive/negative controls is recommended for experimental accuracy.
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