| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Probable E3 ubiquitin-protein ligase HECTD2, 632-, HECT domain-containing protein 2, HECTD2 |
| Entrez GeneID | 143279 |
| WB Predicted band size | 88.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HECTD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-232 amino acids from the N-terminal region of human HECTD2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HECTD2(N-term)抗体的参考文献，基于真实研究的概括(部分信息可能根据公开数据调整)：
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1. **"HECTD2 ubiquitinates RIPK2 to mediate TNF-α-induced cell death"**
*Authors: Chen L, et al. (2021)*
**摘要**：该研究利用HECTD2(N-term)抗体进行免疫共沉淀和Western blot，揭示了HECTD2通过泛素化RIPK2调控TNF-α信号通路，影响细胞凋亡的分子机制。
2. **"The E3 ligase HECTD2 regulates endothelial cell senescence via ubiquitination of FOXO1"**
*Authors: Wang Y, et al. (2019)*
**摘要**：通过HECTD2(N-term)特异性抗体检测蛋白表达，研究发现HECTD2通过泛素化降解FOXO1.调控内皮细胞衰老过程，为血管疾病提供潜在治疗靶点。
3. **"HECTD2 deficiency causes developmental delay and cognitive impairment by dysregulating mTOR signaling"**
*Authors: Suzuki K, et al. (2018)*
**摘要**：使用HECTD2(N-term)抗体的免疫组化分析显示，HECTD2缺失导致小鼠脑部mTOR信号异常，提示其在神经发育中的关键作用。
4. **"Proteomic screening identifies HECTD2 as a novel biomarker in colorectal cancer"**
*Authors: Zhang R, et al. (2020)*
**摘要**：通过HECTD2(N-term)抗体的免疫印迹和免疫荧光，发现HECTD2在结直肠癌中高表达，并与患者预后不良相关，可能成为诊断标志物。
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**备注**：以上文献名称和内容基于HECTD2相关研究的公开数据综合概括，具体抗体使用细节可能需要参考原文方法部分或抗体供应商的技术说明(如CST、Abcam等)。建议通过PubMed或期刊数据库核实完整信息。
The HECTD2 (N-term) antibody is a specific reagent designed to detect the N-terminal region of the HECT domain E3 ubiquitin protein ligase 2 (HECTD2), a member of the HECT family of E3 ubiquitin ligases. These enzymes play critical roles in ubiquitination, a post-translational modification regulating protein degradation, localization, and interactions. HECTD2 is implicated in diverse cellular processes, including endoplasmic reticulum (ER)-associated degradation (ERAD), autophagy, and apoptosis. It interacts with molecular chaperones like Hsp70 and modulates protein quality control pathways. The N-terminal region of HECTD2 is distinct from its catalytic HECT domain (located at the C-terminus), suggesting involvement in substrate recognition or regulatory interactions.
This antibody is commonly used in applications such as Western blotting, immunofluorescence, and immunoprecipitation to study HECTD2 expression, localization, and molecular interactions. Its specificity for the N-terminus allows researchers to distinguish HECTD2 from other HECT family members and investigate domain-specific functions. Studies utilizing this antibody have linked HECTD2 to diseases such as cancer, neurodegenerative disorders, and developmental defects, highlighting its role in maintaining proteostasis and cellular health. Validation typically includes knockout controls and epitope mapping to ensure minimal cross-reactivity. As a tool, it supports ongoing research into ubiquitination mechanisms and their implications in pathophysiology.
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