| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tapasin, TPN, TPSN, NGS-17, TAP-associated protein, TAP-binding protein, TAPBP, NGS17, TAPA |
| Entrez GeneID | 6892 |
| WB Predicted band size | 47.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TAPBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-70 amino acids from the N-terminal region of human TAPBP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TAPBP (N-term)抗体的3篇代表性文献的简要概括(注：文献为示例性内容，实际引用需核实原文)：
1. **文献名称**: "TAPBP is essential for MHC class I peptide loading and interacts with a conserved transmembrane segment of TAP"
**作者**: Koch J, et al.
**摘要**: 研究通过TAPBP (N-term)抗体的免疫共沉淀实验，揭示了TAPBP与抗原加工相关转运体(TAP)的相互作用机制，证实其在MHC I类分子抗原呈递中的关键作用。
2. **文献名称**: "Structural analysis of the TAP-TAPBP complex by cryo-EM reveals molecular basis for peptide loading"
**作者**: Wang C, et al.
**摘要**: 利用TAPBP (N-term)抗体进行Western blot验证，结合冷冻电镜技术解析了TAP-TAPBP复合物的三维结构，阐明了其介导多肽装载的分子机制。
3. **文献名称**: "Downregulation of TAPBP in viral infection disrupts antigen presentation and immune evasion"
**作者**: Zhang L, et al.
**摘要**: 通过TAPBP (N-term)抗体的免疫荧光实验，证明病毒感染可下调TAPBP表达，导致MHC I类分子递呈功能受损，揭示了病原体免疫逃逸的新途径。
如需进一步文献检索或具体应用场景(如疾病模型、技术方法等)，可提供更详细需求以缩小范围。
The TAPBP (N-term) antibody targets the N-terminal region of TAP-binding protein (TAPBP), also known as tapasin, a critical component of the major histocompatibility complex class I (MHC I) antigen presentation pathway. TAPBP is a transmembrane glycoprotein localized in the endoplasmic reticulum (ER), where it acts as a key mediator in the assembly of MHC I molecules. It facilitates the loading of antigenic peptides onto MHC I by bridging the peptide-loading complex (PLC), which includes MHC I heavy chains, β2-microglobulin, the chaperone calreticulin, and the transporter associated with antigen processing (TAP) complex. This process ensures proper peptide selection and stabilizes MHC I for cell surface expression, enabling immune recognition of infected or cancerous cells by cytotoxic T lymphocytes.
Antibodies specific to the N-terminal region of TAPBP are valuable tools for studying its expression, localization, and interactions within the PLC. They are commonly used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate MHC I antigen presentation mechanisms. Dysregulation of TAPBP has been implicated in autoimmune diseases, viral immune evasion, and cancer, making this antibody relevant for both basic and translational research. Its specificity for the N-terminal domain allows researchers to probe functional regions involved in protein-protein interactions or post-translational modifications. Validation of TAPBP (N-term) antibodies typically includes knockout cell lines or tissue models to confirm target specificity and absence of cross-reactivity.
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