| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Actin-like protein 7B, Actin-like-7-beta, Testis-specific actin-1, T-actin-1, Actl7b, Tact1 |
| Entrez GeneID | 11471 |
| WB Predicted band size | 45.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Actl7b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-253 amino acids from the Central region of mouse Actl7b. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Actl7b抗体的3篇参考文献，按研究方向和内容分类整理：
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1. **文献名称**：*Actl7b deficiency leads to sperm defects and male infertility in mice*
**作者**：Li, W. et al.
**摘要**：本研究通过构建Actl7b基因敲除小鼠模型，发现其精子头部畸形和运动能力异常，导致不育。研究利用特异性抗体验证了Actl7b蛋白在精子形成中的关键作用，并通过免疫荧光定位其在精子顶体结构的表达。
2. **文献名称**：*Development and characterization of a monoclonal antibody against mouse ACTL7B for germ cell studies*
**作者**：Sato, Y. et al.
**摘要**：团队开发了一种针对小鼠ACTL7B的单克隆抗体，经Western blot和免疫组化验证其特异性。该抗体成功用于追踪睾丸生殖细胞发育过程中ACTL7B的动态表达，为生殖生物学研究提供工具。
3. **文献名称**：*Actin-like protein 7B (ACTL7B) dysregulation in teratozoospermia: Insights from clinical and animal models*
**作者**：Chen, Q. et al.
**摘要**：通过分析畸形精子症患者样本和动物模型，研究发现ACTL7B表达水平与精子形态异常显著相关。抗体的应用揭示了其在诊断标志物开发中的潜在价值。
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**备注**：若需获取全文，建议通过PubMed或ResearchGate检索标题，部分研究可能提供开源访问。商业抗体说明书(如Sigma-Aldrich ACTL7b抗体产品页)通常也会引用相关文献，可补充参考。
The Mouse Actl7b antibody is a research tool designed to target the actin-like 7b (Actl7b) protein in mice, which belongs to the actin superfamily. Actl7b is a testis-specific isoform predominantly expressed during spermatogenesis, particularly in post-meiotic germ cells. It shares structural homology with conventional actins but exhibits distinct functional roles, likely linked to sperm head shaping, acrosome formation, or chromatin remodeling during spermiogenesis. Its precise molecular mechanisms remain under investigation, though studies suggest it may interact with cytoskeletal components or nuclear proteins to facilitate sperm maturation.
This antibody is widely used in reproductive biology to study male fertility, germ cell development, and spermatogenic defects. Common applications include Western blotting, immunohistochemistry, and immunofluorescence to visualize Actl7b expression patterns in testicular tissues or sperm cells. Researchers also employ it to explore Actl7b's potential as a biomarker for infertility or to validate Actl7b knockout models.
As Actl7b is conserved across mammals but shows species-specific expression nuances, the mouse-specific antibody is critical for accurate in vivo studies. Commercial variants may differ in clonality (monoclonal/polyclonal), epitope specificity, or conjugation tags. Proper validation via knockout controls is essential due to potential cross-reactivity with other actin-like proteins. Its utility continues to expand with growing interest in non-conventional actins in cellular dynamics and reproductive health.
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