| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Short transient receptor potential channel 4-associated protein, Trp4-associated protein, Trpc4-associated protein, Protein TAP1, TNF-receptor ubiquitous scaffolding/signaling protein, Protein TRUSS, TRPC4AP, C20orf188, TRRP4AP |
| Entrez GeneID | 26133 |
| WB Predicted band size | 90.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TRPC4AP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-75 amino acids from the N-terminal region of human TRPC4AP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于TRPC4AP (N-term)抗体的参考文献示例(内容基于模拟生成，实际文献可能需要进一步检索验证)：
---
1. **文献名称**: *Characterization of TRPC4AP Antibodies for Subcellular Localization Studies*
**作者**: Müller A, et al.
**摘要**: 本研究开发并验证了一种针对TRPC4AP蛋白N端表位的多克隆抗体。通过Western blot在HEK293细胞中检测到约75 kDa的特异性条带，免疫荧光显示TRPC4AP主要定位于细胞核内。抗体经siRNA敲低实验验证特异性，并用于探究TRPC4AP在细胞周期中的动态分布。
2. **文献名称**: *TRPC4AP Interaction with NFAT Signaling Pathway in Cardiomyocytes*
**作者**: Chen L, et al.
**摘要**: 本文利用TRPC4AP (N-term)抗体(购自Abcam, cat# ab12345)研究其与钙调神经磷酸酶-NFAT通路的关联。通过免疫共沉淀(Co-IP)证实TRPC4AP与NFAT3在心肌细胞中的相互作用，并发现N端结构域对结合至关重要。抗体特异性通过基因敲除小鼠模型验证。
3. **文献名称**: *Role of TRPC4AP in B Cell Development: Insights from Antibody-Based Knockdown*
**作者**: Sato K, et al.
**摘要**: 研究采用TRPC4AP N端抗体(自主研发)评估其在B细胞分化中的功能。流式细胞术和免疫组化显示TRPC4AP在生发中心B细胞高表达。通过抗体介导的蛋白阻断实验，发现TRPC4AP缺失导致B细胞增殖异常，提示其参与免疫调控。
4. **文献名称**: *TRPC4AP Antibody Validation for Proteomic Profiling in Cancer*
**作者**: Gupta R, et al.
**摘要**: 本文系统评估了多种TRPC4AP抗体的性能，包括N端抗体(Sigma-Aldrich, HPA012345)。通过质谱分析确认抗体识别位点，并应用于结直肠癌组织芯片，发现TRPC4AP表达与患者预后显著相关。强调抗体优化对临床样本分析的重要性。
---
**注**：以上文献信息为模拟示例，实际引用需通过PubMed、Web of Science等平台检索确认。建议使用关键词“TRPC4AP antibody N-terminal”、“TRPC4AP epitope mapping”或结合抗体货号(如已知)进行精确查找。
The TRPC4AP (Transient Receptor Potential Cation Channel Subfamily C Member 4-Associated Protein) N-term antibody is a specialized reagent designed to target the N-terminal region of the TRPC4AP protein, a less characterized but biologically significant molecule. TRPC4AP, also known as TRRAP-interacting protein, is implicated in transcriptional regulation and chromatin remodeling through its interaction with TRRAP, a component of histone acetyltransferase complexes. The protein is structurally characterized by multiple coiled-coil domains, which mediate protein-protein interactions critical for its role in cellular processes like DNA damage response, cell cycle regulation, and apoptosis.
The N-terminal region of TRPC4AP is believed to harbor functional motifs essential for binding partner recruitment or subcellular localization. Antibodies targeting this region are valuable tools for studying TRPC4AP expression, localization, and interaction networks in various experimental settings, including Western blotting, immunofluorescence, and co-immunoprecipitation. These applications help elucidate TRPC4AP's involvement in diseases such as cancer, where dysregulation of chromatin modifiers and transcriptional coactivators is common. Additionally, the antibody aids in distinguishing TRPC4AP isoforms or truncations that may arise from alternative splicing or proteolytic processing. Validation of such antibodies typically includes testing for specificity using knockout cell lines or siRNA-mediated knockdown. Research using TRPC4AP N-term antibodies continues to uncover its potential as a biomarker or therapeutic target in pathological contexts linked to transcriptional dysregulation.
×
