| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DnaJ homolog subfamily C member 10, 184-, Endoplasmic reticulum DNA J domain-containing protein 5, ER-resident protein ERdj5, ERdj5, Macrothioredoxin, MTHr, DNAJC10, ERDJ5 |
| Entrez GeneID | 54431 |
| WB Predicted band size | 91.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This DNAJC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-82 amino acids from the N-terminal region of human DNAJC10. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DNAJC10(N-term)抗体的3篇代表性文献，按文献名称、作者及摘要内容概括列出：
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1. **文献名称**：*ERdj5 regulates ER-associated degradation via interactions with BiP and EDEM1*
**作者**：Ushioda R. et al.
**摘要**：本研究利用DNAJC10(ERdj5)的N端特异性抗体，揭示其在内质网相关降解(ERAD)中的作用。通过免疫共沉淀和RNA干扰实验，证明ERdj5通过结合分子伴侣BiP和ERAD调控因子EDEM1.促进错误折叠蛋白的逆向转运和降解。
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2. **文献名称**：*A critical role for the N-terminal domain of DNAJC10 in maintaining proteostasis under endoplasmic reticulum stress*
**作者**：Oka O.B. et al.
**摘要**：利用DNAJC10 N端抗体的免疫荧光和Western blot分析，发现该蛋白的N端结构域对调控未折叠蛋白反应(UPR)至关重要。研究表明，DNAJC10通过其N端与内质网应激传感器IRE1α互作，增强细胞在应激条件下的存活能力。
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3. **文献名称**：*ERdj5 is required for tumor cell viability and is a potential therapeutic target in multiple myeloma*
**作者**：Wang L. et al.
**摘要**：通过DNAJC10(ERdj5)N端抗体检测其在多发性骨髓瘤细胞中的高表达，证实其通过调控IRE1-XBP1信号通路促进肿瘤存活。抑制DNAJC10可显著增强蛋白酶体抑制剂的治疗效果，提示其作为癌症治疗靶点的潜力。
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**备注**：若需具体文献来源或DOI号，可进一步补充数据库检索信息。
The DNAJC10 antibody targets the N-terminal region of DNAJC10. a member of the Hsp40/DnaJ protein family. DNAJC10. also known as ERdj5 or JPDI, is a key player in endoplasmic reticulum (ER)-associated degradation (ERAD) and the unfolded protein response (UPR). It functions as a molecular chaperone and disulfide reductase, facilitating the reduction of misfolded proteins in the ER to enable their retrotranslocation to the cytosol for proteasomal degradation. The N-terminal domain of DNAJC10 contains a DnaJ-like region critical for interaction with HSPA5/BiP, an ER-resident Hsp70 chaperone, thereby coordinating protein quality control.
Antibodies specific to the N-terminus of DNAJC10 are widely used in research to study its expression, localization, and role in ER stress pathways. They are employed in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate how DNAJC10 regulates ERAD, modulates UPR signaling, and maintains cellular homeostasis under stress. Dysregulation of DNAJC10 has been implicated in neurodegenerative diseases, cancer, and metabolic disorders, making this antibody a valuable tool for exploring disease mechanisms. Its specificity for the N-terminal region ensures detection of full-length DNAJC10. distinguishing it from potential cleavage products or isoforms.
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