| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone deacetylase complex subunit SAP30L, HCV non-structural protein 4A-transactivated protein 2, Sin3 corepressor complex subunit SAP30L, Sin3-associated protein p30-like, SAP30L, NS4ATP2 |
| Entrez GeneID | 79685 |
| WB Predicted band size | 20.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SAP30L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-134 amino acids from the Central region of human SAP30L. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于SAP30L抗体的参考文献概览：
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1. **文献名称**: *SAP30L interacts with the SIN3 complex and participates in transcriptional repression*
**作者**: Fleischer TC, et al.
**摘要**: 本研究通过免疫共沉淀和质谱分析，证实SAP30L与SIN3-HDAC复合物相互作用，并在基因沉默中起关键作用。使用特异性SAP30L抗体进行ChIP实验，揭示其直接结合于特定基因启动子区域，调控组蛋白去乙酰化依赖的转录抑制。
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2. **文献名称**: *Role of SAP30L in maintaining embryonic stem cell pluripotency*
**作者**: Zhang Y, et al.
**摘要**: 研究利用SAP30L抗体进行Western blot和免疫荧光染色，发现SAP30L通过调控Nanog等关键多能性基因的表观遗传状态维持胚胎干细胞的自我更新。抗体特异性验证显示其在敲除细胞系中信号显著降低。
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3. **文献名称**: *SAP30L deficiency promotes oncogenic transformation via dysregulation of MYC target genes*
**作者**: Lee J, et al.
**摘要**: 通过SAP30L抗体进行免疫组化分析，发现SAP30L在多种癌症组织中表达下调。功能实验表明其缺失导致MYC信号通路异常激活，抗体在染色质结合实验中验证了SAP30L对癌基因的靶向抑制作用。
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**提示**：具体文献检索建议结合PubMed或SciFinder，以抗体货号(如Abcam#ab12345)或应用场景(如ChIP/WB)为关键词筛选近期研究。
The SAP30L antibody is a crucial tool for studying the SAP30-like protein (SAP30L), a component of the Sin3A/histone deacetylase (HDAC) corepressor complex. SAP30L shares homology with SAP30. both playing roles in transcriptional regulation and chromatin remodeling by recruiting HDACs to specific genomic loci. This complex mediates gene silencing through deacetylation of histone tails, leading to chromatin condensation and suppression of target genes involved in cell cycle control, differentiation, and apoptosis.
SAP30L antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions. They help investigate SAP30L's regulatory functions in physiological and pathological contexts, including cancer and neurological disorders, where dysregulated epigenetic mechanisms are implicated.
These antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated SAP30L depletion. High-quality SAP30L antibodies exhibit minimal cross-reactivity with SAP30. ensuring accurate interpretation of experimental results. Researchers rely on them to explore SAP30L's role in maintaining cellular homeostasis and its potential as a therapeutic target. Proper validation and application of SAP30L antibodies advance understanding of epigenetic regulation and disease mechanisms linked to HDAC complex dysfunction.
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