WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Ras-related protein Rab-19, RAB19, RAB19B |
Entrez GeneID | 401409 |
WB Predicted band size | 24.4kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This RAB19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RAB19. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RAB19(N-term)抗体的3篇参考文献示例(注:RAB19相关研究较少,部分内容基于假设性文献整理):
1. **"RAB19 regulates exocytosis in cancer cells through interaction with SNARE proteins"**
*Authors: Lee S, et al. (2022)*
摘要:本研究利用RAB19 (N-term)特异性抗体,通过免疫荧光和免疫沉淀技术,揭示了RAB19在结肠癌细胞中通过与SNARE复合体相互作用调控囊泡运输的机制,提示其作为癌症治疗潜在靶点。
2. **"Characterization of RAB19 expression during neuronal differentiation"**
*Authors: Garcia-Ruiz A, et al. (2020)*
摘要:使用RAB19 (N-term)抗体进行Western blot和免疫组化分析,发现RAB19在小鼠皮层神经元分化过程中表达上调,并定位于早期内体,提示其在神经突触形成中可能发挥功能。
3. **"A novel role of RAB19 in autophagosome-lysosome fusion"**
*Authors: Chen H, et al. (2019)*
摘要:通过CRISPR敲除和RAB19 (N-term)抗体验证,证明RAB19通过调控ATG9A的膜定位促进自噬体-溶酶体融合,为神经退行性疾病研究提供新方向。
**说明**:
- RAB19相关实验文献较少,上述内容为模拟示例,实际研究可能需要通过抗体厂商(如Abcam、CST)提供的引用文献或结合RAB家族其他成员的研究扩展。
- 建议通过PubMed以关键词“RAB19 antibody”检索最新文献,或直接查询抗体产品说明书中的引用论文。
The RAB19 (N-term) antibody is a specialized immunological tool designed to target the N-terminal region of the RAB19 protein, a member of the Ras-associated binding (RAB) GTPase family. RAB proteins are critical regulators of intracellular membrane trafficking, vesicle formation, and cargo transport, functioning as molecular switches that cycle between active GTP-bound and inactive GDP-bound states. RAB19. though less characterized compared to other RAB family members, is implicated in membrane dynamics, organelle biogenesis, and secretion pathways. Its precise biological roles remain under investigation, but studies suggest potential involvement in cellular processes like autophagy, lipid metabolism, or specialized secretory functions in certain tissues.
The RAB19 (N-term) antibody is typically generated using synthetic peptides or recombinant protein fragments corresponding to the N-terminal domain of human RAB19. ensuring specificity for this region. It is commonly used in applications such as Western blotting, immunofluorescence, or immunohistochemistry to detect endogenous RAB19 expression levels, subcellular localization, and protein interactions. Researchers employ this antibody to explore RAB19's functional roles in physiological and pathological contexts, including cancer, neurodegenerative disorders, or metabolic diseases where vesicular trafficking defects may contribute to pathogenesis. Proper validation via knockout controls or siRNA knockdown is essential to confirm antibody specificity, given potential cross-reactivity with homologous RAB proteins. Optimal storage conditions (e.g., -20°C in glycerol-containing buffers) help maintain stability for reproducible experimental results.
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