| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HIRA-interacting protein 3, HIRIP3 |
| Entrez GeneID | 8479 |
| WB Predicted band size | 62.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HIRIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-316 amino acids from the Central region of human HIRIP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HIRIP3抗体的3篇示例参考文献(注：部分内容为模拟示例，实际文献需通过学术数据库核实)：
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1. **文献名称**: *HIRIP3 is a DNA repair protein involved in the maintenance of genomic stability*
**作者**: Lambert, J.P. et al.
**摘要**: 本研究报道了一种特异性识别HIRIP3的多克隆抗体的开发，通过免疫印迹和免疫荧光验证其特异性。研究发现HIRIP3在DNA损伤修复中与HIRA复合体协同作用，调控组蛋白H3.3的沉积，影响细胞对电离辐射的应答。
2. **文献名称**: *Functional characterization of HIRIP3 in chromatin remodeling and transcriptional regulation*
**作者**: Sarma, K. et al.
**摘要**: 作者利用HIRIP3抗体进行免疫共沉淀和ChIP实验，证实HIRIP3与HIRA、DAXX等蛋白形成复合体，参与胚胎干细胞分化中的染色质重塑。抗体验证显示HIRIP3在核内富集，且敲低后导致H3.3组蛋白定位异常。
3. **文献名称**: *HIRIP3 antibody validation for developmental biology applications*
**作者**: Johnson, R.L. et al.
**摘要**: 该文献详细描述了兔源HIRIP3单克隆抗体的制备流程，包括抗原设计(针对C端结构域)和验证数据(Western blot显示单一条带，免疫组化定位小鼠脑组织中的HIRIP3)。研究还提示HIRIP3在神经发育中可能起关键作用。
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**注意**：以上为模拟生成的参考文献，实际研究中请通过PubMed、Web of Science等平台检索具体文献。若需真实文献，可尝试关键词 "HIRIP3 antibody" 或 "HIRA-interacting protein 3" 结合相关功能(如染色质重塑、DNA修复)进行搜索。
The HIRIP3 (HIRA-interacting protein 3) antibody is a tool used to study the HIRIP3 protein, which plays a role in chromatin remodeling and transcriptional regulation. HIRIP3 interacts with the HIRA complex, a conserved histone chaperone responsible for depositing the histone variant H3.3 into chromatin. This deposition is critical for epigenetic regulation, gene expression, and cellular processes like DNA repair and development. HIRIP3’s association with HIRA suggests its involvement in maintaining genomic stability, cell cycle progression, and embryonic development, though its precise molecular mechanisms remain under investigation.
Researchers employ HIRIP3 antibodies primarily in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interaction partners. Studies have linked HIRIP3 dysfunction to potential roles in diseases, including cancer and developmental disorders, due to its impact on chromatin dynamics. For example, aberrant H3.3 incorporation has been observed in certain cancers, implicating HIRIP3 and the HIRA complex in tumorigenesis. The antibody’s specificity and reliability are crucial for elucidating HIRIP3’s biological functions and its crosstalk with other chromatin modifiers. Commercial HIRIP3 antibodies are typically validated in model systems, aiding research into gene regulation networks and therapeutic targeting of epigenetic pathways.
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