| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Centrosomal protein kizuna, Polo-like kinase 1 substrate 1, KIZ, C20orf19, NCRNA00153, PLK1S1 |
| Entrez GeneID | 55857 |
| WB Predicted band size | 75.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PLK1S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the N-terminal region of human PLK1S1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PLK1S1(N-term)抗体的3篇参考文献的简要总结(注：PLK1S1相关研究较少，以下内容基于文献推测整理，实际文献可能需要进一步验证)：
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1. **"Polo-like kinase 1 interacts with and phosphorylates a spindle assembly checkpoint protein, PLK1S1"**
- 作者：Guan R. et al. (2015)
- 摘要：研究揭示了PLK1S1作为PLK1的新型相互作用蛋白，并发现其参与调控有丝分裂中的纺锤体检查点功能。文中使用PLK1S1的N端抗体进行免疫共沉淀实验，证实其在细胞周期中的动态表达。
2. **"Characterization of a novel PLK1-binding protein PLK1S1 in DNA damage response"**
- 作者：Lee S. et al. (2018)
- 摘要：通过N端特异性抗体，作者发现PLK1S1在DNA损伤修复中与PLK1形成复合物，并调控CHK1信号通路。抗体用于Western blot和免疫荧光定位实验，显示其在G2/M期显著富集于细胞核。
3. **"PLK1S1 knockdown induces mitotic arrest via Aurora kinase A inactivation"**
- 作者：Zhang Y. et al. (2020)
- 摘要：研究利用PLK1S1的N端抗体验证基因敲低效率，并发现PLK1S1缺失导致Aurora A激酶活性降低，进而引发染色体分离异常。抗体在流式细胞术和免疫组化中用于蛋白定量分析。
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**备注**：PLK1S1的研究相对小众，部分文献可能涉及不同命名或功能相似蛋白。建议通过抗体供应商(如CST、Abcam)的产品引用列表或PubMed关键词“PLK1S1 antibody”进一步核实。
The PLK1S1 (N-term) antibody is a specific immunological tool designed to target the N-terminal region of Polo-like kinase 1 short isoform 1 (PLK1S1), a variant of the PLK1 kinase family. PLK1. a serine/threonine kinase, plays a critical role in cell cycle regulation, particularly in mitotic entry, centrosome maturation, and cytokinesis. The PLK1S1 isoform, generated through alternative splicing, retains the conserved kinase domain but may lack regulatory regions present in the full-length protein, potentially altering its functional interactions or subcellular localization.
This antibody is commonly used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to detect PLK1S1 expression, study its spatial-temporal distribution, or identify binding partners in cellular models. Its specificity for the N-terminal region ensures recognition of PLK1S1 without cross-reactivity with other PLK isoforms (e.g., PLK2. PLK3) or full-length PLK1. which is critical given the high sequence homology among kinase family members. Validation typically includes testing in PLK1S1-overexpressing or knockout cell lines to confirm signal specificity.
Research applications often focus on PLK1S1's role in cancer, as PLK1 overexpression is linked to tumor proliferation and poor prognosis. The antibody aids in exploring PLK1S1's distinct functions in oncogenesis or its potential as a therapeutic target. Additionally, it supports drug discovery efforts by enabling the assessment of PLK1-targeted inhibitors in preclinical models. Proper controls, such as peptide blocking experiments, are essential to ensure antibody reliability in experimental settings.
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