| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FCGR2B;CD32; FCG2; CD32B; FCGR2; IGFR2; FCGR2C; FcRII-c |
| Entrez GeneID | 2213 |
| clone | 2G6A7 |
| WB Predicted band size | 34kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD32B (AA: 43-217) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于GAGE3 (N-term)抗体的模拟参考文献示例(仅供示例参考，非真实文献)：
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1. **文献名称**: *Immunohistochemical detection of GAGE3 N-terminal epitopes in melanoma progression*
**作者**: Müller A, et al.
**摘要**: 研究利用针对GAGE3蛋白N端的单克隆抗体，通过免疫组化技术分析黑色素瘤组织中GAGE3的表达模式。发现GAGE3在转移性病灶中的表达显著高于原发灶，提示其可能作为肿瘤侵袭性的潜在标志物。
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2. **文献名称**: *Development of a GAGE3-specific ELISA assay using N-terminal targeted polyclonal antibodies*
**作者**: Chen L, et al.
**摘要**: 报道了一种基于兔源多克隆抗体的GAGE3 N端ELISA检测方法，验证了其在血清样本中的灵敏度和特异性，并发现卵巢癌患者血清中GAGE3水平与化疗耐药性呈正相关。
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3. **文献名称**: *Structural mapping of GAGE3 epitopes recognized by N-terminal antibodies in gastric cancer*
**作者**: Sato K, et al.
**摘要**: 通过肽段扫描和X射线晶体学解析了GAGE3 N端抗体结合的线性表位(氨基酸残基15-30)，并证明该表位在胃癌细胞系中高度保守，为靶向治疗提供了结构基础。
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4. **文献名称**: *GAGE3 antibody cross-reactivity analysis across cancer-testis antigens*
**作者**: Gupta R, et al.
**摘要**: 评估了多种GAGE家族抗体(包括N端靶向抗体)的特异性，发现部分抗体与GAGE2/4存在交叉反应，强调使用N端特异性抗体可提高GAGE3检测的准确性，尤其在肺癌组织芯片分析中。
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**注**：以上文献为模拟内容，实际引用需查询真实数据库(如PubMed)并核实原文。
The GAGE3 (N-term) antibody targets the N-terminal region of the GAGE3 protein, a member of the GAGE (G antigen) family. This family comprises cancer-testis antigens (CTAs), which are predominantly expressed in germline cells but aberrantly reactivated in various cancers, including melanoma, lung, and ovarian tumors. Their restricted expression in normal tissues (primarily the testis) and immunogenicity make them potential targets for cancer immunotherapy, such as vaccines or CAR-T therapies.
GAGE3. like other GAGE proteins, contains a conserved N-terminal domain and a variable C-terminal region. The N-terminal epitope recognized by this antibody is critical for detecting GAGE3 across isoforms and paralogs due to sequence homology within the family. This antibody is commonly used in research to study GAGE3's role in oncogenesis, including its association with tumor proliferation, immune evasion, and poor prognosis.
Studies suggest GAGE proteins interact with regulatory pathways, such as p53 signaling, and may promote genomic instability or apoptosis resistance. The GAGE3 (N-term) antibody is utilized in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to assess protein expression patterns in cancer tissues or cell lines. Its specificity helps differentiate GAGE3 from other family members, aiding in mechanistic and diagnostic studies. However, cross-reactivity with homologous GAGE proteins requires careful validation in experimental settings.
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