| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger protein 558, ZNF558 |
| Entrez GeneID | 148156 |
| WB Predicted band size | 45.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ZNF558 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-147 amino acids from the Central region of human ZNF558. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZNF558抗体的假设性参考文献示例(注：实际文献可能需要通过学术数据库验证，以下为模拟内容)：
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1. **文献名称**: "ZNF558 modulates Wnt/β-catenin signaling in neural stem cells via direct transcriptional regulation"
**作者**: Smith, J. et al. (2021)
**摘要**: 本研究利用特异性ZNF558抗体，通过染色质免疫沉淀(ChIP)和Western blot技术，揭示了ZNF558通过调控Wnt通路相关基因的表达影响神经干细胞的增殖与分化。抗体验证显示其在人源和小鼠细胞系中具有高特异性。
2. **文献名称**: "Characterization of a novel monoclonal antibody against ZNF558 and its application in glioblastoma diagnostics"
**作者**: Lee, H. et al. (2020)
**摘要**: 研究团队开发了一种靶向ZNF558 C端结构域的单克隆抗体，并通过免疫组化(IHC)和流式细胞术验证其特异性。该抗体成功应用于胶质母细胞瘤组织样本，显示ZNF558表达与肿瘤侵袭性呈正相关。
3. **文献名称**: "ZNF558 interacts with KRAB-associated protein 1 (KAP1) to regulate retrotransposon silencing"
**作者**: Zhang, Y. et al. (2019)
**摘要**: 通过免疫共沉淀(Co-IP)结合ZNF558抗体，研究发现ZNF558与KAP1形成复合物，参与逆转录转座子的表观遗传沉默。抗体在HEK293T细胞模型中的敲除/回补实验证实了其功能依赖性。
4. **文献名称**: "A high-throughput screening platform for ZNF558 inhibitors using antibody-based fluorescence polarization"
**作者**: Johnson, R. et al. (2022)
**摘要**: 研究利用荧光标记的ZNF558抗体建立高通量药物筛选系统，用于识别调控ZNF558-DNA结合的小分子抑制剂。抗体在体外结合实验中表现出高亲和力，为靶向治疗提供工具。
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**注意**：以上文献为模拟生成，实际研究中可能需通过PubMed、Google Scholar或Web of Science等平台检索真实数据。若需具体文献，建议使用关键词“ZNF558 antibody”结合筛选条件(如发表时间、研究领域)进一步查询。
ZNF558 (Zinc Finger Protein 558) is a member of the zinc finger protein family, characterized by conserved C2H2-type zinc finger domains that mediate sequence-specific DNA or RNA binding. It is implicated in transcriptional regulation and chromatin remodeling, though its precise biological functions remain under investigation. Recent studies suggest ZNF558 may act as a transcriptional repressor, potentially influencing cellular processes such as mitochondrial homeostasis, inflammation, and differentiation. Notably, a 2021 study linked ZNF558 to the regulation of *VPS53*, a gene involved in the endosomal sorting complex, and highlighted its role in modulating mitochondrial RNA processing and innate immune signaling. Dysregulation of ZNF558 has been tentatively associated with neurodevelopmental disorders and cancer progression, though mechanistic details are still emerging.
ZNF558 antibodies are immunological tools developed to detect and study the expression, localization, and molecular interactions of ZNF558 in various experimental models. These antibodies, typically raised in rabbit or mouse hosts, enable applications including Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and co-immunoprecipitation (Co-IP). Validation often involves knockout cell lines or tissues to confirm specificity. Commercially available ZNF558 antibodies have facilitated research into its nuclear-cytoplasmic shuttling dynamics, post-translational modifications, and involvement in signaling pathways like NF-κB. However, antibody performance may vary depending on epitope accessibility and cross-reactivity with homologous zinc finger proteins, necessitating careful optimization in experimental setups.
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