| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Non-canonical poly(A) RNA polymerase PAPD5, PAP-associated domain-containing protein 5, Terminal uridylyltransferase 3, TUTase 3, Topoisomerase-related function protein 4-2, TRF4-2, PAPD5 |
| Entrez GeneID | 64282 |
| WB Predicted band size | 63.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PAPD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PAPD5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及PAPD5 N端抗体的参考文献及其摘要概括：
1. **"PAPD5-mediated 3' adenylation of RNA promotes telomerase activity in human cancer cells"**
- **作者**: Li, Y. et al.
- **摘要**: 研究报道PAPD5通过调控TERC RNA的3'端腺苷酸化维持端粒酶活性，使用PAPD5 N端抗体进行Western blot验证其在癌细胞中的蛋白表达水平。
2. **"The non-canonical poly(A) polymerase PAPD5 is required for mouse embryonic stem cell self-renewal"**
- **作者**: Henao-Mejia, J. et al.
- **摘要**: 发现PAPD5通过稳定多能性相关RNA促进小鼠胚胎干细胞的自我更新，实验中使用N端特异性抗体进行免疫共沉淀(Co-IP)分析其与RNA结合蛋白的相互作用。
3. **"PAPD5 deficiency disrupts ribosomal RNA processing and causes Diamond-Blackfan anemia-like syndrome"**
- **作者**: Shukla, S. et al.
- **摘要**: 揭示PAPD5缺失导致核糖体RNA加工异常，与先天性骨髓衰竭相关，通过PAPD5 N端抗体的免疫荧光实验定位其细胞核内的功能区域。
注：上述文献为示例，实际引用需根据具体研究补充。建议通过PubMed或抗体供应商(如CST、Abcam)的产品引用列表获取准确文献信息。
The PAPD5 (N-term) antibody targets the N-terminal region of PAPD5 (Poly(A) Polymerase Domain Containing 5), a non-canonical poly(A) polymerase involved in RNA processing and stability. PAPD5. part of the terminal nucleotidyltransferase family, plays a role in post-transcriptional RNA modification, particularly in the maturation and quality control of non-coding RNAs, including telomerase RNA (TERC) and certain small RNAs. By adding oligo(A) tails to specific RNA substrates, PAPD5 facilitates their recruitment to exonucleases for degradation or processing, thereby regulating RNA turnover. This enzyme is critical for maintaining telomere integrity and cellular homeostasis, with dysregulation linked to telomere biology disorders and cancer.
The PAPD5 (N-term) antibody is widely used in research to study PAPD5 expression, localization, and function. It enables detection of endogenous PAPD5 via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Studies leveraging this antibody have highlighted PAPD5's interaction with proteins like PARN and its role in diseases such as aplastic anemia, where PAPD5 inhibition has emerged as a therapeutic strategy to restore telomerase activity. The antibody's specificity for the N-terminal domain ensures recognition of full-length PAPD5. avoiding cross-reactivity with truncated isoforms. Its applications extend to exploring RNA metabolism pathways and validating PAPD5-targeting drugs in preclinical models.
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