| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | O-phosphoseryl-tRNA(Sec) selenium transferase, Liver-pancreas antigen, LP, SLA-p35, SLA/LP autoantigen, Selenocysteine synthase, Sec synthase, Selenocysteinyl-tRNA(Sec) synthase, Sep-tRNA:Sec-tRNA synthase, SepSecS, Soluble liver antigen, SLA, UGA suppressor tRNA-associated protein, tRNA(Ser/Sec)-associated antigenic protein, SEPSECS, TRNP48 |
| Entrez GeneID | 51091 |
| WB Predicted band size | 55.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SEPSECS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-474 amino acids from the C-terminal region of human SEPSECS. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SEPSECS抗体的3-4篇参考文献示例(文献信息为虚构，仅供参考)：
1. **文献名称**: "SEPSECS mutations impair tRNA modification and cause pontocerebellar hypoplasia"
**作者**: Agamy, O. et al.
**摘要**: 本研究通过免疫印迹(Western blot)和免疫荧光技术，利用SEPSECS特异性抗体发现其基因突变导致患者细胞中SEPSECS蛋白表达显著降低，进而影响硒代半胱氨酸tRNA修饰，引发小脑发育异常。
2. **文献名称**: "Localization and functional analysis of SEPSECS in mammalian cells"
**作者**: Suzuki, T. & Nakajima, K.
**摘要**: 通过亚细胞定位实验(使用SEPSECS抗体进行免疫染色)，发现SEPSECS蛋白主要定位于细胞核周区域，并证实其与硒蛋白合成通路中其他酶类的相互作用。
3. **文献名称**: "SEPSECS deficiency disrupts redox homeostasis and mitochondrial function"
**作者**: Xu, J. et al.
**摘要**: 研究利用SEPSECS抗体进行组织特异性敲除小鼠模型分析，表明SEPSECS缺失导致线粒体氧化应激水平升高，揭示其在维持细胞氧化还原平衡中的关键作用。
4. **文献名称**: "Antibody-based detection of SEPSECS in neurodegenerative disorders"
**作者**: Lee, S. et al.
**摘要**: 开发了一种高特异性SEPSECS单克隆抗体，通过免疫组化验证其在帕金森病患者脑组织中的表达异常，提示其可能与神经退行性病变相关。
(注：以上文献为示例，实际引用需根据具体研究检索PubMed、Google Scholar等数据库获取真实信息。)
SEPSECS (Sep [O-phosphoserine] tRNA:Sec [selenocysteine] synthase) is a crucial enzyme involved in the biosynthesis of selenocysteine (Sec), the 21st amino acid encoded by the UGA codon in selenoprotein mRNAs. It catalyzes the final step of Sec synthesis by converting O-phosphoserine-tRNA(Sec) to selenocysteine-tRNA(Sec), a process essential for incorporating Sec into selenoproteins. These selenoproteins, such as glutathione peroxidases and thioredoxin reductases, play vital roles in antioxidant defense, redox signaling, and thyroid hormone metabolism.
SEPSECS antibodies are valuable tools for studying the expression, localization, and regulation of this enzyme in cellular and pathological contexts. Dysregulation of SEPSECS has been implicated in disorders linked to oxidative stress, including neurodegenerative diseases (e.g., Parkinson’s and Alzheimer’s), cancer, and developmental defects. Research using SEPSECS antibodies has revealed its nuclear-cytoplasmic shuttling behavior and interactions with components of the selenocysteine incorporation machinery.
These antibodies are widely applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate tissue-specific expression patterns or altered SEPSECS levels in disease models. Understanding SEPSECS function through antibody-based studies provides insights into selenoprotein synthesis mechanisms and their implications in human health, offering potential therapeutic targets for selenoprotein-related pathologies.
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