| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9, MED9, MED25 |
| Entrez GeneID | 55090 |
| WB Predicted band size | 16.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This MED9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-136 amino acids from the C-terminal region of human MED9. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MED9抗体的3篇模拟参考文献(基于领域内常见研究方向，建议通过PubMed或Google Scholar核实具体文献)：
1. **文献名称**: *"Mediator complex subunit MED9 is essential for early embryonic development in mice"*
**作者**: Smith A, et al.
**摘要**: 本研究利用特异性MED9抗体，通过免疫沉淀和ChIP-seq技术，揭示了MED9在中介体复合物中对胚胎干细胞多能性基因(如Oct4、Nanog)的转录调控作用，并证明其缺失导致小鼠胚胎早期死亡。
2. **文献名称**: *"Structural and functional analysis of the Mediator tail module reveals MED9’s role in CDK8 kinase activity regulation"*
**作者**: Johnson R, et al.
**摘要**: 通过冷冻电镜和MED9抗体的Western blot验证，文章解析了MED9在中介体尾部模块的结构，发现其与CDK8激酶亚基的相互作用，并影响转录暂停与激活的平衡。
3. **文献名称**: *"Dysregulation of MED9 expression in breast cancer correlates with hormone receptor status"*
**作者**: Chen L, et al.
**摘要**: 使用商业化MED9抗体进行免疫组化分析，发现MED9在雌激素受体阳性乳腺癌中高表达，且其沉默可抑制细胞增殖，提示其作为潜在治疗靶点。
**注意**：以上文献信息为示例性质，实际研究中建议通过学术数据库检索关键词(如“MED9 antibody”、“Mediator subunit 9”)获取最新论文。真实文献可能包括抗体应用(如Abcam、Cell Signaling Technology等公司产品引用文献)。
The MED9 antibody targets the MED9 protein, a critical subunit of the Mediator complex—a multi-protein assembly essential for regulating RNA Polymerase II (Pol II)-mediated transcription. The Mediator complex, composed of approximately 30 subunits, acts as a molecular bridge between transcription factors and the Pol II machinery, facilitating the initiation and modulation of gene expression. MED9. part of the core Mediator module, contributes to structural stability and functional coordination of the complex, influencing processes like cell proliferation, differentiation, and stress responses.
MED9 antibodies are widely used in research to study the Mediator complex's role in transcriptional regulation, chromatin interactions, and disease mechanisms. They enable detection, localization, and quantification of MED9 in various experimental setups, including Western blotting, immunoprecipitation, and immunofluorescence. Dysregulation of MED9 has been implicated in cancers and developmental disorders, making these antibodies valuable tools for exploring pathological pathways.
Validated MED9 antibodies exhibit specificity for their target, often confirmed through knockout controls or siRNA-mediated depletion. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to ensure accurate analysis of MED9's interactions and post-translational modifications. Commercial availability from multiple suppliers supports diverse applications, from basic molecular studies to translational research in oncology and genetics.
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