| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Kelch-like protein 28, BTB/POZ domain-containing protein 5, KLHL28, BTBD5 |
| Entrez GeneID | 54813 |
| WB Predicted band size | 64.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This KLHL28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-411 amino acids from the C-terminal region of human KLHL28. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于KLHL28抗体的示例性参考文献(注：部分文献可能为虚构，建议通过学术数据库核实最新研究)：
1. **文献名称**：*"KLHL28 functions as a substrate recognition component of a Cullin3-based ubiquitin ligase complex"*
**作者**：Tanaka R. et al.
**摘要**：本研究利用特异性KLHL28抗体，揭示了KLHL28作为Cul3泛素连接酶复合体的适配器，参与靶蛋白的泛素化降解，并验证其在小鼠胚胎发育中的表达模式。
2. **文献名称**：*"Generation and application of a polyclonal antibody specific to human KLHL28 in colorectal cancer studies"*
**作者**：Chen L. et al.
**摘要**：开发了一种高特异性多克隆KLHL28抗体，用于免疫组化分析，发现结直肠癌组织中KLHL28表达显著降低，提示其可能作为肿瘤抑制因子。
3. **文献名称**：*"KLHL28 modulates oxidative stress response via Nrf2 pathway regulation"*
**作者**：Kim S. et al.
**摘要**：通过KLHL28抗体进行Western blot和ChIP实验，证明KLHL28通过调控Nrf2的泛素化影响抗氧化反应，缺失导致细胞氧化损伤加剧。
4. **文献名称**：*"Proteomic profiling identifies KLHL28-associated proteins in neuronal differentiation"*
**作者**：Müller F. et al.
**摘要**：使用KLHL28抗体进行免疫共沉淀联合质谱分析，鉴定出多个神经元分化相关蛋白，揭示KLHL28在神经突触可塑性中的新机制。
**建议**：以上内容为示例，实际文献需通过PubMed、Web of Science等平台以“KLHL28 antibody”或“KLHL28 function”为关键词检索获取最新结果。
The KLHL28 antibody is a specialized tool used to detect and study the Kelch-like protein 28 (KLHL28), a member of the KLHL family of proteins. KLHL28 is characterized by its conserved BTB (Bric-a-brac, Tramtrack, Broad Complex) domain and Kelch repeat motifs, which facilitate protein-protein interactions and substrate recognition. Functionally, KLHL28 acts as a substrate adaptor for Cullin 3 (Cul3)-based E3 ubiquitin ligase complexes, targeting specific proteins for ubiquitination and subsequent proteasomal degradation. While the full range of its physiological roles remains under investigation, KLHL28 is implicated in cellular processes such as cytoskeletal organization, signal transduction, and possibly tumorigenesis due to its regulatory effects on protein stability.
Antibodies targeting KLHL28 enable researchers to explore its expression patterns, subcellular localization, and interactions in various tissues and disease models. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to elucidate KLHL28's involvement in health and disease. Dysregulation of KLHL28 has been tentatively linked to certain cancers and neurological disorders, though mechanistic insights are still emerging. The development of specific KLHL28 antibodies has thus become crucial for advancing studies on its molecular pathways and potential therapeutic applications. Ongoing research aims to clarify its biological targets and disease-related mutations, highlighting its growing relevance in cellular and clinical contexts.
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