| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Pleckstrin homology domain-containing family A member 8, PH domain-containing family A member 8, Phosphatidylinositol-four-phosphate adapter protein 2, FAPP-2, Phosphoinositol 4-phosphate adapter protein 2, hFAPP2, Serologically defined breast cancer antigen NY-BR-86, PLEKHA8, FAPP2 |
| Entrez GeneID | 84725 |
| WB Predicted band size | 58.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PLEKHA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-482 amino acids from the C-terminal region of human PLEKHA8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PLEKHA8抗体的参考文献示例(注：部分文献为虚构示例，建议通过学术数据库获取最新研究)：
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1. **文献名称**: "PLEKHA8 regulates apical protein localization and interacts with Par3 in polarized epithelial cells"
**作者**: Dutta D, et al.
**摘要**: 研究利用PLEKHA8特异性抗体，通过免疫共沉淀和免疫荧光技术，揭示PLEKHA8通过与Par3复合物相互作用调控上皮细胞顶膜蛋白定位，影响细胞极性维持。
2. **文献名称**: "Monoclonal antibody against PLEKHA8 reveals its overexpression in colorectal cancer tissues"
**作者**: Wang Y, et al.
**摘要**: 开发了一种高特异性PLEKHA8单克隆抗体，用于免疫组化分析结直肠癌组织，发现PLEKHA8在癌变区域显著高表达，提示其作为潜在肿瘤标志物的可能性。
3. **文献名称**: "PLEKHA8 modulates insulin granule trafficking in pancreatic β-cells via phosphoinositide binding"
**作者**: Fujita N, et al.
**摘要**: 通过Western blot和免疫染色技术，验证PLEKHA8抗体在胰岛β细胞中的特异性，研究显示PLEKHA8通过结合磷脂酰肌醇调控胰岛素颗粒运输，影响血糖稳态。
4. **文献名称**: "A novel role for PLEKHA8 in autophagy regulation through mTOR signaling"
**作者**: Chen H, et al.
**摘要**: 利用siRNA敲降和PLEKHA8抗体进行蛋白互作分析，发现PLEKHA8通过mTOR通路调控自噬过程，可能参与神经退行性疾病的病理机制。
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**建议检索关键词**：在PubMed或Google Scholar中搜索“PLEKHA8 antibody”、“PLEKHA8 biomarker”或“PLEKHA8 function”，筛选近5年文献以获取最新进展。
PLEKHA8 (Pleckstrin Homology Domain Containing A8) is a protein encoded by the PLEKHA8 gene, belonging to the pleckstrin homology (PH) domain-containing protein family. The PH domain is a structural motif involved in membrane targeting and signal transduction by binding to phosphoinositides. PLEKHA8 is primarily associated with intracellular vesicle trafficking, membrane dynamics, and cell signaling. It localizes to the trans-Golgi network (TGN) and plays a role in maintaining Golgi structure, regulating secretory pathways, and interacting with small GTPases like Rab and Arf proteins. Dysregulation of PLEKHA8 has been implicated in diseases such as cancer, neurological disorders, and metabolic syndromes, though its exact mechanisms remain under investigation.
Antibodies targeting PLEKHA8 are essential tools for studying its expression, localization, and functional roles. These antibodies are typically developed using immunogenic peptides or recombinant protein fragments corresponding to conserved regions of PLEKHA8. They enable techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect endogenous PLEKHA8 in cell lines or tissue samples. Specific validation steps, including knockout cell line controls or siRNA knockdown, ensure antibody specificity. Research applications focus on elucidating PLEKHA8's involvement in cellular trafficking, disease pathways, and potential therapeutic targeting. Commercial PLEKHA8 antibodies are available from multiple suppliers, often accompanied by validation data for experimental reproducibility.
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