| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PURH; AICAR; AICARFT; IMPCHASE; HEL-S-70p |
| WB Predicted band size | 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ATIC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LIPT2(N-term)抗体的3篇参考文献示例(注：文献信息为模拟生成，实际文献需通过学术数据库检索确认)：
1. **文献名称**: "Characterization of LIPT2 N-terminal antibody in mitochondrial lipoylation pathways"
**作者**: Smith A, et al.
**摘要**: 该研究验证了LIPT2(N-term)抗体的特异性，证实其在人源细胞线粒体提取物中能有效识别内源性LIPT2蛋白的N端区域，并揭示LIPT2在硫辛酸修饰酶复合体组装中的关键作用。
2. **文献名称**: "LIPT2 deficiency disrupts cellular metabolism: Insights from antibody-based protein profiling"
**作者**: Chen B, et al.
**摘要**: 通过LIPT2(N-term)抗体的免疫印迹和免疫荧光分析，发现LIPT2缺失导致线粒体丙酮酸脱氢酶复合体功能异常，进而影响细胞能量代谢，提示其与代谢疾病的相关性。
3. **文献名称**: "Development of a polyclonal antibody targeting the N-terminus of human LIPT2 for functional studies"
**作者**: Gupta R, et al.
**摘要**: 本文报道了一种新型兔源多克隆抗体的制备与验证，该抗体针对人LIPT2蛋白的N端表位，成功应用于免疫共沉淀(Co-IP)实验，揭示了LIPT2与线粒体伴侣蛋白的相互作用网络。
建议通过 **PubMed** 或 **Google Scholar** 以“LIPT2 antibody N-terminal”为关键词检索最新文献，或查阅抗体供应商(如Abcam、Sigma-Aldrich)产品页引用的参考文献获取具体信息。
The LIPT2 (N-term) antibody is designed to detect the N-terminal region of the human lipoyltransferase 2 (LIPT2) protein, a key enzyme involved in mitochondrial metabolism. LIPT2 facilitates the covalent attachment of lipoyl groups to the E2 subunits of alpha-ketoacid dehydrogenase complexes, such as the pyruvate dehydrogenase complex (PDC), which is critical for glucose homeostasis and energy production. This post-translational modification, known as lipoylation, is essential for the proper function of these multienzyme complexes in the tricarboxylic acid (TCA) cycle. Dysregulation of lipoylation processes has been linked to metabolic disorders, neurodegenerative diseases, and cancer.
The LIPT2 (N-term) antibody serves as a tool for studying LIPT2 expression, localization, and molecular interactions in various tissues and disease models. It is commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation. Researchers employ this antibody to explore LIPT2's role in mitochondrial dysfunction, metabolic reprogramming in cancer, or rare genetic disorders associated with lipoylation defects. Specificity for the N-terminal region ensures recognition of full-length or truncated variants, aiding in the characterization of splice isoforms or degradation products. Validation typically includes knockout controls or siRNA-mediated silencing to confirm target specificity.
This antibody contributes to advancing understanding of cellular energy metabolism and its implications in health and disease.
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